LOCUS       sequence-53384-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, 
            Wach A, Philippsen P, Pringle JR
  TITLE     Heterologous modules for efficient and versatile PCR-based gene 
            targeting in Schizosaccharomyces pombe.
  JOURNAL   Yeast. 1998 Jul;14(10):943-51.
  PUBMED    9717240
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "943-51"
COMMENT     Sequence Label: pFA6a-kanMX6-P3nmt1-GST
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             118..771
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     729..751
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     promoter        complement(847..865)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(848..865)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atataaagga agaggaatcc tggcatatca tcaattgaat aagttgaatt aattatttca
       61 atctcattct cactttctga cttatagtcg ctttgttaaa tcatgtcttt aattaatatg
      121 tcccctatac taggttattg gaaaattaag ggccttgtgc aacccactcg acttcttttg
      181 gaatatcttg aagaaaaata tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg
      241 cgaaacaaaa agtttgaatt gggtttggag tttcccaatc ttccttatta tattgatggt
      301 gatgttaaat taacacagtc tatggccatc atacgttata tagctgacaa gcacaacatg
      361 ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc ttgaaggagc ggttttggat
      421 attagatacg gtgtttcgag aattgcatat agtaaagact ttgaaactct caaagttgat
      481 tttcttagca agctacctga aatgctgaaa atgttcgaag atcgtttatg tcataaaaca
      541 tatttaaatg gtgatcatgt aacccatcct gacttcatgt tgtatgacgc tcttgatgtt
      601 gttttataca tggacccaat gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa
      661 cgtattgaag ctatcccaca aattgataag tacttgaaat ccagcaagta tatagcatgg
      721 cctttgcagg gctggcaagc cacgtttggt ggtggcgacc atcctccaaa atcggatctg
      781 gttccgcgtt gaggcgcgcc agatccgtcg acctgcagcg tacgaagctt cagctggcgg
      841 ccgcgttcta tagtgtcacc taaatcgtat gtgtatgata cataaggt
//
