LOCUS       sequence-53386-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Kim SH, Kim K, Kwagh JG, Dicker DT, Herlyn M, Rustgi AK, Chen Y, 
            El-Deiry WS
  TITLE     Death induction by recombinant native TRAIL and its prevention by a 
            caspase 9 inhibitor in primary human esophageal epithelial cells.
  JOURNAL   J Biol Chem. 2004 Sep 17. 279(38):40044-52.
  PUBMED    15226295
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Sep 17. 279(38):40044-52."
COMMENT     Sequence Label: pQE-nTR
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..30
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(54..68,69..74,75..91,92..98)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    74..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    106..122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             141..152
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      690..784
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 taacctataa aaataggcgt atcacgaggc cctttcgtct tcacctcgag aaatcataaa
       61 aaatttattt gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac
      121 aatttcacac agaattcatt aaagaggaga aattaactat ggtgagagaa agaggtcctc
      181 agagagtagc agctcacata actgggacca gaggaagaag caacacattg tcttctccaa
      241 actccaagaa tgaaaaggct ctgggccgca aaataaactc ctgggaatca tcaaggagtg
      301 ggcattcatt cctgagcaac ttgcacttga ggaatggtga actggtcatc catgaaaaag
      361 ggttttacta catctattcc caaacatact ttcgatttca ggaggaaata aaagaaaaca
      421 caaagaacga caaacaaatg gtccaatata tttacaaata cacaagttat cctgacccta
      481 tattgttgat gaaaagtgct agaaatagtt gttggtctaa agatgcagaa tatggactct
      541 attccatcta tcaaggggga atatttgagc ttaaggaaaa tgacagaatt tttgtttctg
      601 taacaaatga gcacttgata gacatggacc atgaagccag tttttttggg gcctttttag
      661 ttggctaaaa gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc
      721 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
      781 gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
      841 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttggaggc
      901 attt
//
