LOCUS       sequence-53390-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Lim KH, Huang H, Pralle A, Park S
  TITLE     Stable, high-affinity streptavidin monomer for protein labeling and 
            monovalent biotin detection.
  JOURNAL   Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. 
            Epub 2012 Aug 8.
  PUBMED    22806584
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24605";
            journalName: "Biotechnol Bioeng"; date: "2013-01"; volume: "110"; 
            issue: "1"; pages: "57-67"
COMMENT     Sequence Label: pRSET-mSA
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..745)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     CDS             complement(764..787)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gggctttgtt agcagccgga tcaagctttt atttgtacaa ttcattcata ccctcggtaa
       61 taccagcagc agtcaaaaat tctaacaaga ccatgtggtc tctcttttcg tttggatctt
      121 tgaataactt agattgatag gataagtaat ggttgtctgg taacaagact ggaccatcac
      181 caattggagt attttgttga taatggtcag ctaattgaac accaccatct tcaatgttgt
      241 gtctaatttt gaagttagct ttgataccat tcttttgttt gtcagcagtg atgtaaacat
      301 tgtgagagtt atagttgtat tccaatttgt gacctaaaat gttaccatct tctttaaaat
      361 caataccttt taattcgatt ctattaacta aggtatcacc ttcaaacttg acttcagctc
      421 tggtcttgta gttaccgtca tctttgaaaa aaatagttct ttcttgaaca taaccttctg
      481 gcatggcaga cttgaaaaag tcatgttgtt tcatatgatc tgggtatcta gcaaaacatt
      541 gaacaccata acctaaagta gtgactaagg ttggccatgg aactggcaat ttaccagtag
      601 tacagagtaa ttttaaggtc aatttaccgt acgtagcatc accttcacct tcaccggaga
      661 cagaaaattt gtgaccatta acatcaccat ctaattcaac caaaattggg acaacaccag
      721 tgaataattc ttcaccttta gacatagatc cgccctcgag ttacttgtcg tcatcgtcct
      781 tgtagtcgga tccagacgcc gcagacggtt taactttggt g
//
