LOCUS       sequence-53395-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of EBFP2 blue fluorescent protein with NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE
  TITLE     Exploration of New Chromophore Structures Leads to the 
            Identification of Improved Blue Fluorescent Proteins.
  JOURNAL   Biochemistry. 2007 May 22;46(20):5904-10. doi: 10.1021/bi700199g
  PUBMED    17444659
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochemistry"; date: "2007-05-22- 22"; volume: "46"; issue: "20"; 
            pages: "5904-10"
COMMENT     Sequence Label: pEBFP2-Nuc
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(134..154)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(158..178)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(182..202)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      121 atccggtgga tcctaccttt ctcttctttt ttggatctac ctttctcttc ttttttggat
      181 ctacctttct cttctttttt ggatcagctc gagatctgag tccggacttg tacagctcgt
      241 ccatgccgag agtgatcccg gcggcggtgc ggaactccag caggaccatg tgatcgcgct
      301 tctcgttggg gtctttgctc agcacggact gggtgctcag gtagtggctg tcgggcagca
      361 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
      421 cgtcctccac gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgacgg
      481 ccatgatata gatgttgtgg ctgttgaagt tgtactccag cttgtgcccc aggatgttgc
      541 cgtcctcctt gaagtcgacg cccttcagct cgatgcggtt cactagggtg tcgccctcga
      601 acttcacctc ggcgcgggtc ttgtaggtgc cgtcgtcctt gaagaagatg gtgcgctcct
      661 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
      721 agcgggcgaa gcactgcacg ccgtggctca
//
