LOCUS       sequence-53405-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(W583del)
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..49)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    102..126
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cggtacgcgt agaatcgaga ccgaggagag ggttagggat aggcttacct tcgaaccgcg
       61 ggccctctag actcgagcgg ccgccactgt gctggatatc aaccactttg tacaagaaag
      121 ctgggtcggc gcgcccaccc ttaattaatc atcgcctctg ctgtgcatat actgggtagg
      181 ctagtgtgac attgagggag tcgttgtgct gcacaaggga ggtgtgttgg taatgtagca
      241 ccagttcttt cagagagctg tacaagttat agggctcggc aaagccatag ccagttgctg
      301 ttttgtttat gacacaatgc tttacttcgc cgtccaccac tacagagcag gcatagcagc
      361 cctgtttact gctctcccgg acaagaaaag tgccatctcg cttccctcgc aacaggtttt
      421 cagctttgtt tcggttgctg cttccaacat tccatgtctt ctcatcatga tggggcaaat
      481 cttcatcatc ttccaccagt gaatattggt cttcagtgtt ttcattgccc aaccactcgt
      541 tcaacttctt ttgccgaaca cctttttgag tcaacatcaa gtattggtct ctcgtctttc
      601 tcagctggat aaggtctggt ttaatgctgt tcatacgttt gtcaatttct cgatactcag
      661 ctgcctgctt cttcaagtct tcttccaatc ttcttctact gtcaataatt tcactgattc
      721 gagacttcaa cttatcataa ttatgcataa tcctttgtat ttctttctca ttgccttcac
      781 gtttaaactt ttctatgtat tctttgctgt accgctcttg ggtctggcac tgttcttcaa
      841 atatttttat ggtttcatta aatgcttcaa tagctgtcct tttcatttgg atttcctggg
      901 atgtgcgggt atattcttca tataatctat catattctcg actttttctt gaaac
//
