LOCUS       sequence-53409-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ31f
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    126..147
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      194..223
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      379..473
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
       61 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
      121 aataactcta gaagcggccg cgaattcgag tcactaaggg ctaactaact aattacgtag
      181 caatcaactc actggctcac cttcacgggt gggcctttct tcggcacggg caaattgctg
      241 aatattcctt ttcttagacg tcaggtggca aacgcgaagt aatcttttcg gttttaaaga
      301 aaaagggcag ggtggtgaca ccttgcccgt ttttttgccg gagctagagc tgtcagacca
      361 agtttacgag ctcgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
      421 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagca
      481 ctagggacag taagacgggt aagcctgttg atgataccgc tgccttactg ggtgcattag
      541 ccagtctgaa tgacctgtca cgggataatc cgaagtggtc agactggaaa atcagagggc
      601 aggaactgct gaacagcaaa aagtcagata gcaccacata gcagacccgc cataaaacgc
      661 cctgagaagc ccgtgacggg cttttcttgt attatgggta gtttccttgc atgaatccat
      721 aaaaggcgcc tgtagtgcca tttaccccca ttcactgcca gagccgtgag cgcagcga
//
