LOCUS       sequence-53411-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ11-12
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             161..172
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(198..225)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(241..312)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    408..426
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    433..451
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    496..512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             559..570
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cacctttggt aactttcagt ttagcggtct gggtaccttc gtacggacga ccttcacctt
       61 caccttcgat ttcgaactcg tgaccgttaa cggaaccttc catacgaact ttgaaacgca
      121 tgaactcttt gataacgtct tcggaggaag ccatctagta tttctcctct ttctctagtg
      181 gatccgctag ctataaacgc agaaaggccc acccgaaggt gagccagtgt gactctagta
      241 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
      301 tcgttttatt tgatgcctgg cctagggcct cgcataaatc tgtcacagca tcaacgtttc
      361 cgctgaactg tgacagactc acacaatcaa ccctcccgtg ctcagtatct ctatcactga
      421 tagggatgtc aatctctatc actgataggg aatgcatgct aggatgcata taaatgtgag
      481 cggataacat tgacattgtg agcggataac aagatactga gcacacccag tatcattctc
      541 ttcttcctgc ccacgcggaa agaggagaaa ggtgtaatga ctagccgtaa aggcgaagag
      601 ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa cggtcataag
      661 ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac gctgaagttc
      721 atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac gctgacttat
      781 g
//
