LOCUS       sequence-53415-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Wullschleger S, Loewith R, Oppliger W, Hall MN
  TITLE     Molecular organization of target of rapamycin complex 2.
  JOURNAL   J Biol Chem. 2005 Sep 2;280(35):30697-704. Epub 2005 Jul 7.
  PUBMED    16002396
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-09-2"; volume: "280(35)"; pages: "30697-704. 
            Epub 2005 Jul 7"
COMMENT     Sequence Label: pHAC111-LST8 (pSW75)
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    344..365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(380..385,386..403,404..410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    418..434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     423..445
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     442..458
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     442..458
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(477..724)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     690..708
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      301 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      361 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      421 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcaa
      481 attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg ttttcagtat aatgttacat
      541 gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa atataaataa cgttcttaat
      601 actaacataa ctataaaaaa ataaataggg acctagactt caggttgtct aactccttcc
      661 ttttcggtta gagcggatgt ggggggaggg cgtgaatgta agcgtgacat aactaattac
      721 atgatatcga caaaggaaaa ggggcctgtt tactcacagg cttttttcaa gtaggtaatt
      781 aagtcgtttc tgtctttttc cttcttcaac ccaccaaagg ccatcttgg
//
