LOCUS       sequence-53422-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00574
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             707..724
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagttgg caccatggtc atgttgcaat cgccggcgca
      121 aaaggccagc gatagtgcgt cagcccaaaa cacagccgtc ggtggcctga tgagtcccaa
      181 ctcgaacccg gactcgccca agtccaacac ctcgccggat gtggcgagtg cggacagtgt
      241 ggtgtccggc acaggcggtg gcagcactcc gccagcggcc aagataccca agttcatcat
      301 cagcgccaat ggcgcagcgg tggcgggaaa acaggagcag gagctgaggt actccctcga
      361 acggctcaag cagatgagca gtgaatcggg cagcttgctc agccgcttga gccccttgca
      421 ggaggactcc caggataagg agaaacccaa tcacaacaac aacagcagtc taactaatca
      481 taatgcaaac agcaatacac gtcgctcgca atcccctcca gcttcgatgg gatctgtaag
      541 cttctcctcg ccagcccagc agcgaaagct cctggaactg aatgctgtgc gccacttggc
      601 aaggccggag ccactgcagc atcctcatgc ggccctgctg cagcagcatc cccacttgct
      661 gcagaatccg cagttcctgg ccgccgccca gcagcacatg caccatcatc agcatcagca
      721 tcagcatcac cagcatcccg cacatccgca ttcgcatcag catccgcatc cacaccctca
      781 tccgcatccc catccgcatc cgtcggcggt ctttcatttg agggctccca gcagcagcag
      841 cactgccc
//
