LOCUS       sequence-53425-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Shin J, Noireaux V
  TITLE     Efficient cell-free expression with the endogenous E. Coli RNA 
            polymerase and sigma factor 70.
  JOURNAL   J Biol Eng. 2010 Jun 24;4:8.
  PUBMED    20576148
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2010-06-24"; volume: "4"; pages: "8"
COMMENT     Sequence Label: pBEST-OR2-OR1-Pr-UTR1-deGFP-T500
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..230
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     RBS             267..289
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(627..645)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        713..817
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
       61 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      121 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      181 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggaaca
      241 acgccagtga aaagctccat ggtatatctc cttcttaaag ttaaacaaaa ttattgctag
      301 ctgcaaccat tatcaccgcc agaggtaaaa ttgtcaacac gcacggtgtt agctcagcat
      361 gcccagcgga acagcacaca catgtcgcgg tggttacgtc cgtcacgttc acgcatcagg
      421 cggatatcgt tagcccaccc agcaaaattc ggttttctgg ctgatggtgc gatagtcttc
      481 accatgtcaa acatccactc tgcggcggtc aggtcttctg ctgtccccca cttgctggcg
      541 ctctgaattg cagcatccgg tttcaccaca gaaaggtcgt tttctggctg gtcagaggat
      601 tcgccagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
      661 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      721 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      781 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      841 cgcccttatt
//
