LOCUS       sequence-53428-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, 
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, 
            Bonneau R, Selbach M, Dieterich C, Landthaler M
  TITLE     The mRNA-Bound Proteome and Its Global Occupancy Profile on 
            Protein-Coding Transcripts.
  JOURNAL   Mol Cell. 2012 Jun 8;46(5):674-90.
  PUBMED    22681889
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2012-06-8"; volume: "46(5)"; pages: "674-90"
COMMENT     Sequence Label: pFRT/TO/HIS/FLAG/HA-PGK1
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             172..195
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             199..222
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             223..249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    265..289
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccat gcaccaccac
      181 caccaccacc accacatgga ctacaaggac gacgatgaca agtaccctta tgacgtgccc
      241 gattacgctt tgaaccagat atcaacaagt ttgtacaaaa aagcaggctc caccatggga
      301 accatgtcgc tttctaacaa gctgacgctg gacaagctgg acgttaaagg gaagcgggtc
      361 gttatgagag tcgacttcaa tgttcctatg aagaacaacc agataacaaa caaccagagg
      421 attaaggctg ctgtcccaag catcaaattc tgcttggaca atggagccaa gtcggtagtc
      481 cttatgagcc acctaggccg gcctgatggt gtgcccatgc ctgacaagta ctccttagag
      541 ccagttgctg tagaactcaa atctctgctg ggcaaggatg ttctgttctt gaaggactgt
      601 gtaggcccag aagtggagaa agcctgtgcc aacccagctg ctgggtctgt catcctgctg
      661 gagaacctcc gctttcatgt ggaggaagaa gggaagggaa aagatgcttc tgggaacaag
      721 gttaaagccg agccagccaa aatagaagct ttccgagctt cactttccaa gctaggggat
      781 gtctatgtca atgatgcttt tggcactgct cacagagccc acagctccat ggtaggagtc
      841 aatctgccac agaaggctgg tggg
//
