LOCUS       sequence-53430-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, 
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, 
            Bonneau R, Selbach M, Dieterich C, Landthaler M
  TITLE     The mRNA-Bound Proteome and Its Global Occupancy Profile on 
            Protein-Coding Transcripts.
  JOURNAL   Mol Cell. 2012 Jun 8;46(5):674-90.
  PUBMED    22681889
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2012-06-8"; volume: "46(5)"; pages: "674-90"
COMMENT     Sequence Label: pFRT/TO/HIS/FLAG/HA-API5
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             172..195
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             199..222
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             223..249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    265..289
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccat gcaccaccac
      181 caccaccacc accacatgga ctacaaggac gacgatgaca agtaccctta tgacgtgccc
      241 gattacgctt tgaaccagat atcaacaagt ttgtacaaaa aagcaggctc caccatgccg
      301 acagtagagg agctttaccg caattatggc atcctggccg atgccacgga gcaagtgggc
      361 cagcataaag atgcctatca agtgatattg gatggtgtga aaggtggtac taaggaaaag
      421 cgattagcag ctcaatttat tccgaaattc tttaagcatt ttccagaatt ggctgattct
      481 gctatcaatg cacagttaga cctctgtgag gatgaagatg tatctattcg acgtcaagca
      541 attaaagaac tgcctcaatt tgccactgga gaaaatcttc ctcgagtggc agatatacta
      601 acgcaacttt tgcagacaga tgactctgca gaatttaacc tagtgaacaa tgccctatta
      661 agtatattta aaatggatgc aaaagggact ttaggtgggt tgttcagcca aatacttcaa
      721 ggagaggaca ttgttagaga acgagcaatt aaattccttt ctacaaaact taagacttta
      781 ccagatgaag tcttaacaaa ggaagtggaa gagcttatac taactgaatc caaaaaggtc
      841 ctagaagatg tgactggtga agaatttgtt ctatttatga agatactgtc tgg
//
