LOCUS       sequence-53431-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, 
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, 
            Bonneau R, Selbach M, Dieterich C, Landthaler M
  TITLE     The mRNA-Bound Proteome and Its Global Occupancy Profile on 
            Protein-Coding Transcripts.
  JOURNAL   Mol Cell. 2012 Jun 8;46(5):674-90.
  PUBMED    22681889
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2012-06-8"; volume: "46(5)"; pages: "674-90"
COMMENT     Sequence Label: pFRT/TO/HIS/FLAG/HA-BTF3
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             172..195
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             199..222
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             223..249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    265..289
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(785..809)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccat gcaccaccac
      181 caccaccacc accacatgga ctacaaggac gacgatgaca agtaccctta tgacgtgccc
      241 gattacgctt tgaaccagat atcaacaagt ttgtacaaaa aagcaggctc caccatgaaa
      301 gaaacaatca tgaaccagga aaaactcgcc aaactgcagg cacaagtgcg cattggtggg
      361 aaaggaactg ctcgcagaaa gaagaaggtg gttcatagaa cagccacagc agatgacaaa
      421 aaacttcagt tctccttaaa gaagttaggg gtaaacaata tctctggtat tgaagaggtg
      481 aatatgttta caaaccaagg aacagtgatc cactttaaca accctaaagt tcaggcatct
      541 ctggcagcga acactttcac cattacaggc catgctgaga caaagcagct gacagaaatg
      601 ctacccagca tcttaaacca gcttggtgcg gatagtctga ctagtttaag gagactggcc
      661 gaagctctgc ccaaacaatc tgtggatgga aaagcaccac ttgctactgg agaggatgat
      721 gatgatgaag ttccagatct tgtggagaat tttgatgagg cttccaagaa tgaggcaaac
      781 taggacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcaagt
      841 ctagagggcc cgcggttcga aggtaagcct atccct
//
