LOCUS       sequence-53466-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Micutkova L, Hermann M, Offterdinger M, Hess MW, Matscheski A, 
            Pircher H, Muck C, Ebner HL, Laich A, Ferrando-May E, Zwerschke W, 
            Huber LA, Jansen-Durr P
  TITLE     Analysis of the cellular uptake and nuclear delivery of insulin-like
            growth factor binding protein-3 in human osteosarcoma cells.
  JOURNAL   Int J Cancer. 2012 Apr 1;130(7):1544-57. doi: 10.1002/ijc.26149. 
            Epub 2011 Aug 2.
  PUBMED    21520041
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/ijc.26149";
            journalName: "Int J Cancer"; date: "2012-04-1- 1"; volume: "130"; 
            issue: "7"; pages: "1544-57"
COMMENT     Sequence Label: pmCherry-NLS
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(139..159)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(163..183)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(187..207)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(507..526)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ataacagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
       61 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta
      121 tctagatccg gtggatccta cctttctctt cttttttgga tctacctttc tcttcttttt
      181 tggatctacc tttctcttct tttttggatc agctcgagat ctgagtccgg acttgtacag
      241 ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt
      301 gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag
      361 ctgcacgggc ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc
      421 cttcagcttc agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg
      481 ctcggaggag gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa
      541 gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg
      601 ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc
      661 ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga
      721 gccgtacatg aactgagggg acaggatgtc
//
