LOCUS       sequence-53468-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, 
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, 
            Bonneau R, Selbach M, Dieterich C, Landthaler M
  TITLE     The mRNA-Bound Proteome and Its Global Occupancy Profile on 
            Protein-Coding Transcripts.
  JOURNAL   Mol Cell. 2012 Jun 8;46(5):674-90.
  PUBMED    22681889
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2012-06-8"; volume: "46(5)"; pages: "674-90"
COMMENT     Sequence Label: pFRT/TO/HIS/FLAG/HA-PRDX1
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..24
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    27..45
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     55..79
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             179..202
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             206..229
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             230..256
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    272..296
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg
       61 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      121 acaccgggac cgatccagcc tccggactct agcgtttaaa cttaagcttg gtaccatgca
      181 ccaccaccac caccaccacc acatggacta caaggacgac gatgacaagt acccttatga
      241 cgtgcccgat tacgctttga accagatatc aacaagtttg tacaaaaaag caggctccac
      301 catgtcttca ggaaatgcta aaattgggca ccctgccccc aacttcaaag ccacagctgt
      361 tatgccagat ggtcagttta aagatatcag cctgtctgac tacaaaggaa aatatgttgt
      421 gttcttcttt taccctcttg acttcacctt tgtgtgcccc acggagatca ttgctttcag
      481 tgatagggca gaagaattta agaaactcaa ctgccaagtg attggtgctt ctgtggattc
      541 tcacttctgt catctagcat gggtcaatac acctaagaaa caaggaggac tgggacccat
      601 gaacattcct ttggtatcag acccgaagcg caccattgct caggattatg gggtcttaaa
      661 ggctgatgaa ggcatctcgt tcaggggcct ttttatcatt gatgataagg gtattcttcg
      721 gcagatcact gtaaatgacc tccctgttgg ccgctctgtg gatgagactt tgagactagt
      781 tcaggccttc cagttcactg acaaacatgg ggaagtgtgc ccagctggct ggaaacctgg
      841 cagtgatacc atcaagcctg atgtccaaaa gagcaaagaa tatttctcc
//
