LOCUS       sequence-53469-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, 
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, 
            Bonneau R, Selbach M, Dieterich C, Landthaler M
  TITLE     The mRNA-Bound Proteome and Its Global Occupancy Profile on 
            Protein-Coding Transcripts.
  JOURNAL   Mol Cell. 2012 Jun 8;46(5):674-90.
  PUBMED    22681889
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2012-06-8"; volume: "46(5)"; pages: "674-90"
COMMENT     Sequence Label: pFRT/TO/HIS/FLAG/HA-SART1
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             172..195
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             199..222
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             223..249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    265..289
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccat gcaccaccac
      181 caccaccacc accacatgga ctacaaggac gacgatgaca agtaccctta tgacgtgccc
      241 gattacgctt tgaaccagat atcaacaagt ttgtacaaaa aagcaggctc caccatgggg
      301 tcgtccaaga agcatcgcgg agagaaggag gcggccggga cgacggcggc ggccggcacc
      361 gggggtgcca ccgagcagcc gccgcggcac cgggaacaca aaaaacacaa gcaccggagt
      421 ggcggcagtg gcggtagcgg tggcgaacga cggaagcgga gccgggaacg tgggggcgag
      481 cgcgggagcg ggcggcgcgg ggccgaagct gaggcccgga gcagcacgca cgggcgggag
      541 cgcagccagg cagagccctc cgagcggcgc gtgaagcggg agaagcgcga tgacggctac
      601 gaggccgctg ccagctccaa aactagctca ggcgatgcct cctcactcag catcgaggag
      661 actaacaaac tccgggcaaa gttggggctg aaacccttgg aggttaatgc catcaagaag
      721 gaggcgggca ccaaggagga gcccgtgaca gctgatgtca tcaaccctat ggccttgcga
      781 cagcgagagg agctgcggga gaagctggca gctgccaagg agaagcgcct gctgaaccaa
      841 aagctgggga agataaa
//
