LOCUS       sequence-53474-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pDDYFP-A
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..91
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..127
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(131..139,140..154)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(303..321)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     571..590
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     765..788
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tttttcgcac tctctactgt ttctccatac ccgttttttg ggctaacagg aggaattaac
       61 catggggggt tctcatcatc atcatcatca tggtatggct agcatgactg gtggacagca
      121 aatgggtcgg gatctgtacg acgatgacga taaggatccg agctcgagaa tggtgagcaa
      181 gagcgaggag gtcatcaaag agttcatgcg cttcaaggtg cacttggagg gctccgtgaa
      241 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      301 caccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
      361 acagatctgc tacggctcca aggcgtacgt gaagcacccc gccgacgtcc ccgattacat
      421 aaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgcacttcg aggacggcgg
      481 tctggtgacc gtgactcagg acacctccct gcaggacggc acgctgatct acaaggtgaa
      541 gatgcgcggc actaacttcc cccccgacgg ccccgtaatg cagaagaaga ccctgggctg
      601 ggattattcc accgagcgcc tgtaccccga agacggcgtg ctgaagggcg agattctggg
      661 gcgcctgaag ctgaaggacg gcggcctcaa cctggtggag ttcaagacca tctacatggc
      721 caagaagccc gtgcaactgc ccggctacta cttcgtggac accaagctgg acatcacctc
      781 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
      841 ggggcatggt acaggtagca caggcagcgg tagctccggt acctaactgt tttggcggat
      901 gagaaaaaat
//
