LOCUS       sequence-53475-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pDDYFP-B
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..97
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             101..133
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(137..145,146..160)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     583..602
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gagcttttta tcgcactctc tactgtttct ccatacccgt tttttgggct aacaggagga
       61 attaaccatg gggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg
      121 acagcaaatg ggtcgggatc tgtacgacga tgacgataag gatccgagcg gtaccgcctc
      181 ctccgaggac aacaacatgg ccaccatcaa agagttcatg cgcttccagg tgcgcatgga
      241 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      301 gggcacccag accgccaaac tgaaggtgac caagggcggc cccctgccct tcgcctggga
      361 catcctgtcc ccccagatca tgtacggctc caaagcgtac gtgaggcacc ccgccgacat
      421 ccccgattac aagaagctgc ccttccccga gggcttcagg tgggagcgcg tgatgaactt
      481 cgaagacggc ggtctggtga ccgttaccca ggactcctcc ctgcaggacg gcacgctggt
      541 ctgcaaggtg aagattcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa
      601 gaccatgggc tgggaggcct ccaccgagat gctgtacccc aaagacggcg tgctgaaggg
      661 ccatagctat caggccctga agctgaagga cggcggccac tacccggtgg agttcgagac
      721 catctacatg gccaagaagc ccgtgcaact gcccggcgat tactgtgtgg tcaccaagct
      781 ggtcatcacc tcccacaacg
//
