LOCUS       sequence-53479-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pTOM20-ddGFP-B
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(700..718)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtgccgcc accatggcct cgagaatggt gggtcggaac agcgccatcg
      121 ccgccggtgt atgcggggcc cttttcattg ggtactgcat ctacttcgac cgcaaaagac
      181 gaagtgaccc caacttcaag aacaggcttc gagaacgaag aaagaaacag aagcttgcca
      241 aggagagagc tgggctttcc aagttacctg accttaaaga tgctgaagct gttcagaagt
      301 tcttccttga agaaatacag cttggtgaag agttactagc tcaaggtgaa tatgagaagg
      361 gcgtagacca tctgacaaat gcaattgctg tgtgtggaca gccacagcag ttactgcagg
      421 tcttacagca aactcttcca ccaccagtgt tccagatgct tctgactaag ctcccaacaa
      481 ttagtcagag aattgtaagt gctcagagct tggctgaaga tgatgtggaa ggcggtagcg
      541 gggatccacc ggtcggtacc gcctcctccg aggacaacaa catggccacc atcaaagagt
      601 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
      661 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      721 gcggccccct gccctttgcc tgggacatcc tgtcccccca gatcatgtac ggctccaaag
      781 cgtacgtgag gcacccc
//
