LOCUS       sequence-53489-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Hedgepeth CM, Conrad LJ, Zhang J, Huang HC, Lee VM, Klein PS
  TITLE     Activation of the Wnt signaling pathway: a molecular mechanism for 
            lithium action.
  JOURNAL   Dev Biol. 1997 May 1. 185(1):82-91.
  PUBMED    9169052
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            1997 May 1. 185(1):82-91."
COMMENT     Sequence Label: GSK3beta/pET29b
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagcttgtc
       61 gacggagctc gaattcggat cctcgataca tgtggccagt gttttgagat gggagtccag
      121 ggacagggtg agtggcaccc cctctctctg ttgcccaacc ccccttgcta ctttttgccc
      181 gttttcccgt gggcggcgca tgcttgttgg ctcaggagga gttggaggca gatgcagagg
      241 ctgcgttgtt ggtcgacccg cgctccccag tgtttgaatc tgatgtcgat gttgtatttg
      301 atgttgtgga cacagcggcc tggttgcgag cgtgggccgg gatgagaatt gaggagagag
      361 aaggattgct tgacagctct tgagtggtga agttgaagag agcggggaat tcgcgtccgt
      421 tgggaagttt cagattgggg tcccgcagtt cgtcgaagaa ggagtgggca caggcatcca
      481 ggggggtgag gcgggaggtc ggggtgtatt ccagcagtcg gctgcacaga gcgatggcct
      541 cgggaggggt gcgtgctcgg aacaccttag tccaaggatg cgccttgatc tgtgggaact
      601 tgaactcggt gtagttgggg ttcatttctc ggatctgttc tcgagtcggg gtacccagga
      661 ccttgattat ctcaaccagc tgatccaccc cgctgtcccc cgggaagatg ggctgaccca
      721 gcagcagttc agccaataca cagcctgctg accacacgtc aatactggag gtgtaatctg
      781 tggctccaaa gatcaactcc ggggccctgt agtaccggga gcagat
//
