LOCUS       sequence-53490-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00752
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(664..681)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agttggcaac gtggatatgg acaggttcga
      121 tatctgcaga tcttccgaat tgaggcgcag tatctgctga tcctctgagt ttatgtgcac
      181 caactgcccg ctatcgaagc taagcagatt gctgagcgtt ggcccattat tatccgcttg
      241 attgccagcg gtattattat tgttattatt gttattaagg ttggcattgt tgttggtggg
      301 cgcagtattg tcgtactgct gctgctgctg ctgctgaagc tgctgctgct gatgaagcgt
      361 atgctgattg tgcagatgat ttgtggtcag gttccactgc agattgttgg cgttgccagg
      421 acctccgccc gatgtcagca ggttgtgcat ggtgaaggga ttgttgagat tgctgagatt
      481 attgctattg ccgttggcag gtgctgctcc tgtcgcagtt gctgccgctg ctgcaacagc
      541 tgccgccgag aatttacttt cccagctatt gccgccagga ttgccaaagg gattggcatg
      601 aaattgcaag gactgttgct gttgctgctg ctgctgctgc tgctgctgct gttggtgttg
      661 ctgctggtgc tgatgctggt gctgttgctg ctgctgctga tgctgctgct gctg
//
