LOCUS       sequence-53493-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NNMT
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             926..943
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg ttccgcgtgg tagtatggaa tcaggcttca
      121 cctccaagga cacctatcta agccatttta accctcggga ttacctagaa aaatattaca
      181 agtttggttc taggcactct gcagaaagcc agattcttaa gcaccttctg aaaaatcttt
      241 tcaagatatt ctgcctagac ggtgtgaagg gagacctgct gattgacatc ggctctggcc
      301 ccactatcta tcagctcctc tctgcttgtg aatcctttaa ggagatcgtc gtcactgact
      361 actcagacca gaacctgcag gagctggaga agtggctgaa ggcagcgcca gcggcctttg
      421 actggtcccc agtggtgacc tatgtgtgtg atcttgaagg gaacagagtc aagggtccag
      481 agaaggagga gaagttgaga caggcggtca agcaggtgct gaagtgtgat gtgactcaga
      541 gccagccact gggggccgtc cccttacccc cggctgactg cgtgctcagc acactgtgtc
      601 tggatgccgc ctgcccagac ctccccacct actgcagggc gctcaggaac ctcggcagcc
      661 tactgaagcc agggggcttc ctggtgatca tggatgcgct caagagcagc tactacatga
      721 ttggtgagca gaagttctcc agcctccccc tgggccggga ggcagtagag gctgctgtga
      781 aagaggctgg ctacacaatc gaatggtttg aggtgatctc gcaaagttat tcttccacca
      841 tggccaacaa cgaaggactt ttctccctgg tggcgaggaa gctgagcaga cccctgtgat
      901 gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      961 caaagcccga aaggaagc
//
