LOCUS       sequence-53494-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     yeast Hbs1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28b(+)_S. cerevisiae Hbs1(166-611)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tgagtaattc cctctgaata ttttgtttac tttaagaagg agatatacca tgggcagcag
       61 ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggctagcat
      121 gcctcactta agttttgttg ttcttggtca tgttgatgcg ggaaaatcaa ctctaatggg
      181 cagactactt tatgatctga acattgtcaa ccaatcccaa ctgagaaagc tacaaagaga
      241 gagtgaaact atgggtaagt catccttcaa atttgcatgg attatggatc agacaaatga
      301 agagcgcgaa cgtggtgtaa cagtgtcaat ttgtacatcg catttctcta cccatagggc
      361 aaattttact attgtggatg cgccaggcca tagagatttt gttccaaacg ctataatggg
      421 gatatcacaa gcagatatgg ctatactttg tgtagactgt agcaccaatg ctttcgaatc
      481 aggatttgac ttggatgggc aaacaaagga gcatatgcta ctggcatcta gtcttggaat
      541 tcacaatctg attattgcta tgaataagat ggataatgtt gactggtccc aacaaaggtt
      601 tgaagagatt aaatcgaaat tgttgccata tctggttgat attgggtttt ttgaggataa
      661 tattaattgg gtacctatca gtggcttttc cggagaaggt gtttataaaa ttgaatatac
      721 agatgaagtg agacaatggt acaatggtcc taacttaatg tctactttgg aaaatgcggc
      781 gttcaagatt tcaaaggaga
//
