LOCUS       sequence-53495-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     yeast Hbs1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28b(+)_S. cerevisiae Hbs1(1-611)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..72
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag cggcctggtg
       61 ccgcgcggca gccatatggc tagcatggct tacagtgact acagcgatgg agcagacgac
      121 atgccggact ttcacgacga aggtgaattt gatgattatt tgaatgacga tgaatatgat
      181 ctaatgaacg aggtatttcc tactctaaag gcgcagttac aggattacca aggctgggat
      241 aatctttcac ttaagctagc tttgtttgac aacaatttcg atttggaaag cacgctggcg
      301 gaattgaaga aaactttgaa gaagaagaag acaccaaaga agccaattgc tgctgctaat
      361 gggagtgcga atgttactca aaaactggca aacatctcaa tttcgcaaca aaggccaaat
      421 gatcggcttc cagactggct tgacgaggaa gaaagtgagg gtgaacgcaa tggtgaggaa
      481 gccaatgatg aaaagacagt tcaaagatac tataagacta cggtgccaac gaaaccaaag
      541 aaacctcatg atatttctgc atttgttaaa tctgccttac ctcacttaag ttttgttgtt
      601 cttggtcatg ttgatgcggg aaaatcaact ctaatgggca gactacttta tgatctgaac
      661 attgtcaacc aatcccaact gagaaagcta caaagagaga gtgaaactat gggtaagtca
      721 tccttcaaat ttgcatggat tatggatcag acaaatgaag agcgcgaacg tggtgtaaca
      781 gtgtcaattt gtacatcgca
//
