LOCUS       sequence-53533-e       13893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Tett AJ, Rudder SJ, Bourdes A, Karunakaran R, Poole PS
  TITLE     Regulatable vectors for environmental gene expression in 
            alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2012 Oct;78(19):7137-40. Epub 2012 Jul 20.
  PUBMED    22820336
REFERENCE   2  (bases 1 to 13893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2012-10"; volume: "78(19)"; pages: 
            "7137-40. Epub 2012 Jul 20"
COMMENT     Sequence Label: pLMB51
FEATURES             Location/Qualifiers
     source          1..13893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3289..3305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3289..3305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3302..3324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3313..3329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3337..3343,3344..3361,3362..3367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3382..3403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3531..3549)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            3594..3702
                     /note="incP origin of transfer"
     promoter        3885..3989
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3990..4058,4059..4847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITIADNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4208..4227)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(5370..6569)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             6675..7325
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      7814..8517
                     /label=oriV
                     /note="incP origin of replication"
     CDS             9184..10332
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLMHEEIVVQFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10380..12191)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
ORIGIN
        1 ccggactctg actacaaccg tgcgcaaggc gcattaggga ggatgtatgt atgtaatcgc
       61 ctgcggcatc gttgccggct tggcggctgc ggtggccctg ttgggcttca cgccgatgat
      121 ggaggcgctt gccgccggcg aacgccgcaa ggcactcgcg caatggacgc ggacgatgtt
      181 cctggtgctg ctgcctgtcg tgctgatgtg cgcgcccatc gggtccagca tttacgacgc
      241 cgtgcaagcg gacgctggca agcccatcgc tttccacaac ggccggatca cggtcgtcat
      301 ggccctggtc ggcagcttgg ccgttgtcct ggtcgcggct gcgcgtgcgg tggtcaaccg
      361 caagcatgcc agcttctggt tcgtcggctg ggtgatggcg tcggttttgg ccggcggcgt
      421 cggcgcgatc gccagcgcga agcaactggc gttcctcggc gaacatagcg gcatggtggc
      481 cttcggcttc ttccgcgacc aggtgaagga catgcactgc gatgcggacg tgatcctggc
      541 ccggtgggat gaaaaggcga actcgccggt ggtctaccgc tgcccgaagg cgtacctgct
      601 caacaggttc gcatccgcgc ccttcgtgcc ctggccggac tacaccgagg gggaaagcga
      661 ggatctaggt agggcgctcg cagcggccct gcgggacgcg aaaaggtgag aaaagccggg
      721 cactgcccgg ctttattttt gctgctgcgc gttccaggcc gcccacactc gtttgacctg
      781 gctcgggctg catccgacca gcttggccgt cttggcaatg ctcgatccgc cggagcgaag
      841 cgtgatgatg cggtcgtgca tgccggcgtc acgtttgcgg ccggtgtagc ggccggcggc
      901 cttcgccaac tggacaccct gacgttgacg ctcgcgccga tcctcgtagt cgtcgcgggc
      961 catctgcaag gcgagcttca aaagcatgtc ctggacggat tccagaacga ttttcgccac
     1021 tccgttcgcc tcggcggcca gctccgacag gtccaccacg ccaggcacgg ccagcttggc
     1081 ccctttggcc cggatcgacg caaccaggcg ctcggcctcg gccaacggca agcggctgat
     1141 gcggtcgatc ttctccgcaa cgacgacttc accaggttgc aggtccgcga tcatgcgcag
     1201 cagctcgggc cggtcggcgc gtgcgccgga cgccttctcg cggtagatgc cggcgacgta
     1261 gtacccggcg gcccgcgtgg ccgctacaag gctctcctgg cgttcaagat tctgctcgtc
     1321 cgtactggcg cgcaggtaga tgcgggcgac cttcaacctt cgtccctccg gttgttgctc
     1381 tcgcgtcgcc atttccacgg ctcgacggcg tgcggatcgg accagaggcc gacgcgcttg
     1441 cctcgcgcct cctgttcgag ccgcagcatt tcagggtcgg ccgcgcggcc gtggaagcga
     1501 taggcccacg ccatgccctg gtgaaccatc gcggcgttga cgttgcgcgg ctgcggcggc
     1561 cggctggcca gctccatgtt gacccacacg gtgcccagcg tgcggccgta acggtcggtg
     1621 tccttctcgt cgaccaggac gtgccggcgg aacaccatgc cggccagcgc ctggcgcgca
     1681 cgttcgccga aggcttgccg cttttccggc gcgtcaatgt ccaccaggcg cacgcgcacc
     1741 ggctgcttgt ctaccagcac gtcgatggtg tcgccgtcga tgatgcgcac gacctcgccg
     1801 cgcagctcgg cccatgccgg cgaggcaacg accaggacgg ccagcgcggc agcggcgcgc
     1861 agcatggcgt agcttcggcg cttcatgcgt ggccccattg ctgatgatcg gggtacgcca
     1921 ggtgcagcac tgcatcgaaa ttggccttgc agtagccgtc cagcgccacc cgcgagccga
     1981 acgccggcga aaggtactcg accaggccgg gccggtcgcg gacctcgcgc cccaggacgt
     2041 ggatgcgccg gccgcgtgtg ccgtcgggtc caggcacgaa ggccagcgcc tcgatgttga
     2101 agtcgatgga tagaagttgt cggtagtgct tggccgccct catcgcgtcc cccttggtca
     2161 aattgggtat acccatttgg gcctagtcta gccggcatgg cgcattacag caatacgcaa
     2221 tttaaatgcg cctagcgcat tttcccgacc ttaatgcgcc tcgcgctgta gcctcacgcc
     2281 cacatatgtg ctaatgtggt tacgtgtatt ttatggaggt tatccaatga gccgcctgac
     2341 aatcgacatg acggaccagc agcaccagag cctgaaagcc ctggccgcct tgcagggcaa
     2401 gaccattaag caatacgccc tcgaacgtct gttccccggt gacgctgatg ccgatcaggc
     2461 atggcaggaa ctgaaaacca tgctggggaa ccgcatcaac gatgggcttg ccggcaaggt
     2521 gtccaccaag agcgtcggcg aaattcttga tgaagaactc agcggggatc gcgcttgacg
     2581 gcctacatcc tcacggctga ggccgaagcc gatctacgcg gcatcatccg ctacacgcgc
     2641 cgggagtggg gcgcggcgca ggtgcgccgc tatatcgcta agctggaaca gggcatagcc
     2701 aggcttgccg ccggcgaagg cccgtttaag gacatgagcg aactctttcc cgcgctgcgg
     2761 atggcccgct gcgaacacca ctacgttttt tgcctgccgc gtgcgggcga acccgcgttg
     2821 gtcgtggcga tcctgcatga gcgcatggac ctcatgacgc gacttgccga caggctcaag
     2881 ggctgatttc agccgctaaa aatcgcgcca ctcacaacgt cctgatggcg tacttaccca
     2941 aagaacagct aggagaatca tttatgctca gcacacttcc acaagctcat gcaactttct
     3001 tgaaccgcat ccgcgatgcg gtcgcttccg atgttcgctt ccgcgctctt ctgatcggcg
     3061 gctcttacgt tcacggagga ctcgatgagc actccgattt ggatttcgac atcgttgttg
     3121 aggacaactg ctacgcagat gtcttgtcta cacgcaagga ttttgccgag gcactgcccg
     3181 gcttcctcaa cgcgttcacc ggcgaacatg taggagaacc gcgccttctg atctgcctat
     3241 atggtccgcc actgctacac atcgggggat cagcttggcg taatcatggt catagctgtt
     3301 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3361 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3421 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3481 ggggagaggc ggtttgcgta ttgggcgaat taattcttga agacgaaagg gcctcgtgat
     3541 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagtcga atacggccag
     3601 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
     3661 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgccc ggctgacgcc
     3721 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga
     3781 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatagcagcg
     3841 gaaagcagtc gaccacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3901 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3961 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4021 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4081 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4141 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4201 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     4261 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4321 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatc gctgataaca
     4381 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4441 acaacatggg ggatcatgta acacgccttg atcgttggga accggagctg aatgaagcca
     4501 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4561 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4621 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4681 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccggatg
     4741 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4801 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattgggta actgtcagac
     4861 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4921 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4981 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5041 cgggggatca ggaccgctcc cggagcgcaa cccactcact acagcagagc catgtagaca
     5101 acatcccctc cccctttcca ccgcgtcaga gccccgtagc gccccctacg ggctttttca
     5161 tgccctgccc tagcgtccaa gcctcacgcc gcgctcggcc tctctggcgg ccttctggcg
     5221 ctcctgctgc ggcgtccgct cgtgggccgc ggcgggtccg cgcgccggcc tcgtgcgctg
     5281 gcgctcgcgg gcgaggtcca aggcggccgt cttcacgttc tgccttgcgc agatgagata
     5341 gatccgtcaa ttccctatcg tttccacgat cagcgatcgg ctcgttgccc tgcgccgctc
     5401 caaagcccgc gacgcagcgc cggcaggcag agcaagtaga gggcagcgcc tgcaatccat
     5461 gcccacccgt tccacgttgt tatagaagcc gcatagatcg ccgtgaagag gaggggtccg
     5521 acgatcgagg tcaggctggt gagcgccgcc agtgagcctt gcagctgccc ctgacgttcc
     5581 tcatccacct gcctggacaa cattgcttgc agcgccggca ttccgatgcc acccgaagca
     5641 agcaggacca tgatcgggaa cgccatccat ccccgtgtcg cgaaggcaag caggatgtag
     5701 cctgtgccgt cggcaatcat tccgagcatg agtgcccgcc tttcgccgag ccgggcggct
     5761 acagggccgg tgatcattgc ctgggcgagt gaatgcagaa tgccaaatgc ggcaagcgaa
     5821 atgccgatcg tggtcgcgtc ccagtgaaag cgatcctcgc cgaaaatgac ccaaagcgcg
     5881 gccggcacct gtccgacaag ttgcatgatg aagaagaccg ccatcagggc ggcgacgacg
     5941 gtcatgcccc gggcccaccg gaacgaagcg agcgggttga gagcctcccg gcgtaacggc
     6001 cggcgttcgc ctttgtgcga ctccggcaaa aggaaacagc ccgtcaggaa attgaggccg
     6061 ttcaaggctg ccgcggcgaa gaacggagcg tggggggaga aaccgcccat cagcccaccg
     6121 agcacaggtc ccgcgaccat cccgaacccg aaacaggcgc tcatgaagcc gaagtgccgc
     6181 gcgcgctcat cgccatcagt gatatcggca atataagcgc cggctaccgc cccagtcgcc
     6241 ccggtgatgc cggccacgat ccgcccgata tagagaaccc aaaggaaagg cgctgtcgcc
     6301 atgatggcgt agtcgacagt ggcgccggcc agcgagacga gcaagattgg ccgccgcccg
     6361 aaacgatccg acagcgcgcc cagcacaggt gcgcaggcaa attgcaccaa cgcatacagc
     6421 gccagcagaa tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc gcgcaggagg
     6481 cccggcagca ccggcataat caggccgatg ccgacagcgt cgagcgcgac agtgctcaga
     6541 attacgatca ggggtatgtt gggtttcacg tctggcctcc ggaccagcct ccgctggtcc
     6601 gattgaacgc gcggattctt tatcactgat aagttggtgg acatattatg tttatcagtg
     6661 ataaagtgtc aagcatgaca aagttgcagc cgaatacagt gatccgtgcc gccctggacc
     6721 tgttgaacga ggtcggcgta gacggtctga cgacacgcaa actggcggaa cggttggggg
     6781 ttcagcagcc ggcgctttac tggcacttca ggaacaagcg ggcgctgctc gacgcactgg
     6841 ccgaagccat gctggcggag aatcatacgc attcggtgcc gagagccgac gacgactggc
     6901 gctcatttct gatcgggaat gcccgcagct tcaggcaggc gctgctcgcc taccgcgatg
     6961 gcgcgcgcat ccatgccggc acgcgaccgg gcgcaccgca gatggaaacg gccgacgcgc
     7021 agcttcgctt cctctgcgag gcgggttttt cggccgggga cgccgtcaat gcgctgatga
     7081 caatcagcta cttcactgtt ggggccgtgc ttgaggagca ggccggcgac agcgatgccg
     7141 gcgagcgcgg cggcaccgtt gaacaggctc cgctctcgcc gctgttgcgg gccgcgatag
     7201 acgccttcga cgaagccggt ccggacgcag cgttcgagca gggactcgcg gtgattgtcg
     7261 atggattggc gaaaaggagg ctcgttgtca ggaacgttga aggaccgaga aagggtgacg
     7321 attgatcagg accgctgccg gagcgcaacc cactcactac agcagagcca tgtagacaac
     7381 atcccctccc cctttccacc gcgtcagacg cccgtagcag cccgctacgg gctttttcat
     7441 gccctgccct agcgtccaag cctcacggcc gcgctcggcc tctctggcgg ccttctggcg
     7501 ctcctgctgc ggcgtccgct cgtgggccgt ggcgcgggtc cgcgcgccgg cctcgtgcgc
     7561 ctggcgctcg cgggcgaggt ccagggcggc cgtcttcacg ttctgccttg cgcagatgag
     7621 atagatcgaa ttgacggatc tagcgtggac tcaaggctct cgcgaatggc tcgcgttgga
     7681 aactttcatt gacacttgag gggcaccgca gggaaattct cgtccttgcg cagaaccggc
     7741 tatgtcgtgc tgcgactcga ngcctgcgcc cttggcttgt ctcgcccctc tccgcgtcgc
     7801 tacggggctt ccagcgcctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg
     7861 gcggccgtct atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac
     7921 cgcggccgcc ggcgttgtgg ataccacgcg gaaaacttgg ccctcactga cagatgaggg
     7981 gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag
     8041 gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga
     8101 ttttacgcga gtttcccaga catgatgtgg acaagcctgg ggataagtgc cctgcggtat
     8161 tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg
     8221 cagagtgatg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga
     8281 aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa
     8341 cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctggcgcgt
     8401 gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggct aacgcgggcc
     8461 tcccatcccc ccggctgcgc ccttcggccg cgaacggcct caccccaaaa atggcagcgc
     8521 tggcagtcct tgccattgcc gggatcgggg cagtaacggg atgggcgatc agcccgagcg
     8581 cgacgcccgg aagcattgac gtgccgcagg tgctggcatc gacattcagc gaccaggtgc
     8641 cgggcagtga gggcggcggc ctgggtggcg gcctgccctt cacttcggcc gtcggggcat
     8701 tcacggactt catggcgggg cgggcaattt ttaccttggg cattcttggc atagtggtcg
     8761 cgggtgccgt gctcgtgttc gggggtgaat taattccccg gatcgatccg tcagcttcac
     8821 gctgccgcaa gcactcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca
     8881 gtccgcagaa acggtgctga ccccggatga atgtcacgta ctgggctatc tggacaaggg
     8941 aaaacgcaag cgcaaacaca aacgacctag cttgcagtgg gcttacatgg cgatagctag
     9001 actgggcggt tttatggcca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     9061 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     9121 gcaggggatc aagatcgacg gatcgatccg gggaattccg gggcaatccc gcaaggaggg
     9181 tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt
     9241 tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc
     9301 ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg
     9361 caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctc
     9421 gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg
     9481 accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag
     9541 gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat
     9601 attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg
     9661 ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac
     9721 gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa
     9781 ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc
     9841 ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg
     9901 aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc
     9961 gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag
    10021 aaaacgtccc gttgccaggt cctgatgcac gaggaaatcg tcgtgcagtt tgctggcgac
    10081 cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg
    10141 ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc
    10201 atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc
    10261 gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat
    10321 tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc cacctgcagt
    10381 cattgtttgc ctccctgctg cggtttttca ccgaagttca tgccagtcca gcgtttttgc
    10441 agcagaaaag ccgccgactt cggtttgcgg tcgcgagtga agatcccttt cttgttaccg
    10501 ccaacgcgca atatgccttg cgaggtcgca aaatcggcga aattccatac ctgttcaccg
    10561 acgacggcgc tgacgcgatc aaagacgcgg tgatacatat ccagccatgc acactgatac
    10621 tcttcactcc acatgtcggt gtacattgag tgcagcccgg ctaacgtatc cacgccgtat
    10681 tcggtgatga taatcggctg atgcagtttc tcctgccagg ccagaagttc tttttccagt
    10741 accttctctg ccgtttccaa atcgccgctt tggacatacc atccgtaata acggttcagg
    10801 cacagcacat caaagagatc gctgatggta tcggtgtgag cgtcgcagaa cattacattg
    10861 acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag tggcgcgaaa
    10921 tattcccgtg caccttgcgg acgggtatcc ggttcgttgg caatactcca catcaccacg
    10981 cttgggtggt ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg cgcttgctga
    11041 gtttccccgt tgactgcctc ttcgctgtac agttctttcg gcttgttgcc cgcttcgaaa
    11101 ccaatgccta aagagaggtt aaagccgaca gcagcagttt catcaatcac cacgatgcca
    11161 tgttcatctg cccagtcgag catctcttca gcgtaagggt aatgcgaggt acggtaggag
    11221 ttggccccaa tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt atcgaatcct
    11281 ttgccacgca agtccgcatc ttcatgacga ccaaagccag taaagtagaa cggtttgtgg
    11341 ttaatcagga actgttcgcc cttcactgcc actgaccgga tgccgacgcg aagcgggtag
    11401 atatcacact ctgtctggct tttggctgtg acgcacagtt catagagata accttcaccc
    11461 ggttgccaga ggtgcggatt caccacttgc aaagtcccgc tagtgccttg tccagttgca
    11521 accacctgtt gatccgcatc acgcagttca acgctgacat caccattggc caccacctgc
    11581 cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt gatatcgtcc
    11641 acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggattccggc atagttaaag
    11701 aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac cattcccggc
    11761 gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac acttttcccg
    11821 gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg atgctccatc
    11881 acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa acgcagcacg
    11941 atacgctggc ctgcccaacc tttcggtata aagacttcgc gctgatacca gacgttgccc
    12001 gcataattac gaatatctgc atcggcgaac tgatcgttaa aactgcctgg cacagcaatt
    12061 gcccggcttt cttgtaacgc gctttcccac caacgctgat caattccaca gttttcgcga
    12121 tccagactga atgcccacag gccgtcgagt tttttgattt cacgggttgg ggtttctaca
    12181 ggacggacca tgattacctc agtctagagc tcgagaagct tggatccatg gtaccctaca
    12241 tgtcgttacc ctctttggtt atgtcgcccc gctttttgcg gggtcgcgcc gtacattgaa
    12301 tgacggtctt tgcttgctga atagaaatag gcccacgctt cggattgcct tatatccaga
    12361 taggaagtta gatgagtcca gttgggcgag gtgaacaatg gcgggctcga ccgtctccga
    12421 aacgatcttt ttccttgacc gggccagcca aacgggcctg caggcgcaga tccgcgaaac
    12481 gatcgtttcg gccgtattgt cgggccgcct ggcgccgggc gcccaattgc cctccagccg
    12541 caagcttgcg gcctatctga atatttcgcg gatcaccgta acgctcgcct atcaggaact
    12601 cgcttcccag ggctatgtgg aggcggcgaa ccggagcggc taccgtgtgg ccggcaagcc
    12661 cccaacgaca gccatcgcga tgcaggccgc ccatgctgcg gccgatgcca tcgactggtc
    12721 ggccaagttg cgctcgacct tcatcgtggc caagcagatg cgtaagcccc tcgactggcg
    12781 aacctaccct tatccgttcc tatacggtca gatggacccg tcgctgttcg acctcacggc
    12841 ctggcgcgac tgcgcgcgac gggcgctggc gcgcgaggat ttcgagctga tggcgggcga
    12901 cttcgcggcg gcggacgaca tccagcttgt caactatatc tgctcgcgca cattgccggg
    12961 ccgcggcatc cgcgcgaacc ccgacgagat cctggtgacg gtgggcgcgc agaacgccct
    13021 ctggatcgtc atccagctcc tgttgcgtca gggcgcccat gcggtctgcg agaacccctg
    13081 ccatcccgac atgagtgcat cgcttcgctt gagcggcgcc gggatcacct tcgtcgacgt
    13141 ggacaaggac ggcctgccgc ccgccgcgct tccagaagag gtcgatgccg tgttcgtcac
    13201 gcccagccat cattcgccga ccggcgcgac gatgccgatc gaacggcggg cggccttgct
    13261 cgaggcggcc gcctccaagg acttcataat cgtcgaggac gattacgagt tcgagatgag
    13321 ctttctggcg cccccctccc cggccctcaa ggccttcgac cgctgcggcc gggtcttcta
    13381 catcggcagt ttctccaagt cgttgtttcc cggcctgcgt ctcggctatc tggtggcgcc
    13441 ggctgccgtg atccgggagg cgcgggcact acgggccttg atgttgcggc atccgccggg
    13501 gcacctccag agaacggccg cctatttcct ggcgctcgga cactatgatg ccgtgctgca
    13561 ccgcatgcgc gaggagtatc atcgccgcca catcatcatg gacgctgccc tccgggatgc
    13621 cggattggag atcgccggat ctgcggcttt cggcggcacg tccttctgga tcgaggggcc
    13681 ggaaggtctc gatgcggact tgctgatgaa cgctttgcga aaggacggcg tgctgatcga
    13741 atccgggtcg cccttcttcc cgaaggacga cggcccatgc cgcttcttcc gcatgggcta
    13801 ctcgtcgatc gcgcgcaacc gcatagccga cggcgtggcc ctgactgcgg cgcggatcgc
    13861 ggagcggggt tagaatgagc cttgcagagg tac
//
