LOCUS       sequence-53537-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pDDGFP-B
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..93
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             97..129
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(133..141,142..156)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(311..329)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     579..598
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     773..796
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ttttatcgca actctctact gtttctccat acccgttttt tgggctaaca ggaggaatta
       61 accatggggg gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag
      121 caaatgggtc gggatctgta cgacgatgac gataaggatc cgagcggtac cgcctcctcc
      181 gaggacaaca acatggccac catcaaagag ttcatgcgct tcaaggtgcg catggagggc
      241 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      301 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgccctttgc ctgggacatc
      361 ctgtcccccc agatcatgta cggctccaaa gcgtacgtga ggcaccccgc cgacatcccc
      421 gattacaaga agctgccctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgaa
      481 gacggcggtc tggtgaccgt tacccaggac tcctccctgc aggacggcac gctgatctgc
      541 aaggtgaaga ttcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
      601 atgggctggg aggcctccac cgagatgctg taccccaagg acggcgtgct gaagggccat
      661 agctatcagg ccctgaagct gaaggacggc ggccactacc tggtggagtt cgagaccatc
      721 tacatggcca agaagcccgt gcaactgccc ggcgattact gtgtggacac caagctggac
      781 atcacctccc acaacgagga ctacaccatc gtggaacagt acgggcgct
//
