LOCUS       sequence-53539-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Yoon JY, Kim J, Lee SJ, Kim HS, Im HN, Yoon HJ, Kim KH, Kim SJ, Han 
            BW, Suh SW
  TITLE     Structural and functional characterization of Helicobacter pylori 
            DsbG.
  JOURNAL   FEBS Lett. 2011 Dec 15;585(24):3862-7. Epub 2011 Nov 2.
  PUBMED    22062156
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS 
            Lett."; date: "2011-12-15"; volume: "585(24)"; pages: "3862-7. Epub 
            2011 Nov 2"
COMMENT     Sequence Label: pET21a(+)-HpDsbG C162A
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    795..819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(819..838)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(820..838)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcctt ataatggtat
       61 aagaaaggca cacccttaat cacgccagat tcaaaaatct ttttagtggt attttccact
      121 ttagtgcgta aatcttcagg ctctttttga gcgttaatat cgtattgggt ggaataaatc
      181 ttttcaagaa tagagatctt atcttccacg ctcgctcccc tagccctagc tttcgccatt
      241 tcttcttgga ttaaagccgc ttttttagct gaattgaccc caagccaccc caccacgacc
      301 attctcacgg tgttttcttt taaatgatcc ctaagtttag tgagctcttt ttgagcatgt
      361 gggcacatgg gatcagagac aatataaagg attttatcct tatttgcagc gttagtagag
      421 ggcaactcta tcgcataatc agccggtatt tcattaaaaa tagcgttcaa tttcgcgcta
      481 ttttgttggg tggcgttaag agcttgaact ttttgattgg tttctgcaac taattgcaca
      541 tcatcgcttt tattgctaaa gaatatgttg ctaagcccta tgattaaatt accatcctta
      601 ctcaccacaa gcgggatatt gtatttagtg tccggatctt caataacgac catttttaaa
      661 tcctggctag attttaaagg tttgatttct aaaatacgca cctttttatt ggtctgtttt
      721 tcaatcacgc tcaccaaatt atcctgcata tgtatatctc cttcttaaag ttaaaacaaa
      781 attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat
      841 ttcgcgggat cgagatctcg atcctctacg ccggacgcat cggggg
//
