LOCUS       sequence-53540-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Yoon JY, Kim J, Lee SJ, Kim HS, Im HN, Yoon HJ, Kim KH, Kim SJ, Han 
            BW, Suh SW
  TITLE     Structural and functional characterization of Helicobacter pylori 
            DsbG.
  JOURNAL   FEBS Lett. 2011 Dec 15;585(24):3862-7. Epub 2011 Nov 2.
  PUBMED    22062156
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS 
            Lett."; date: "2011-12-15"; volume: "585(24)"; pages: "3862-7. Epub 
            2011 Nov 2"
COMMENT     Sequence Label: pET21a(+)-HpDsbG
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             766..788
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    805..829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(829..848)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(830..848)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(936..955)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ccttcgggct tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcctta
       61 taatggtata agaaaggcac acccttaatc acgccagatt caaaaatctt tttagtggta
      121 ttttccactt tagtgcgtaa atcttcaggc tctttttgag cgttaatatc gtattgggtg
      181 gaataaatct tttcaagaat agagatctta tcttccacgc tcgctcccct agccctagct
      241 ttcgccattt cttcttggat taaagccgct tttttagctg aattgacccc aagccacccc
      301 accacgacca ttctcacggt gttttctttt aaatgatccc taagtttagt gagctctttt
      361 tggcaatgtg ggcacatggg atcagagaca atataaagga ttttatcctt atttgcagcg
      421 ttagtagagg gcaactctat cgcataatca gccggtattt cattaaaaat agcgttcaat
      481 ttcgcgctat tttgttgggt ggcgttaaga gcttgaactt tttgattggt ttctgcaact
      541 aattgcacat catcgctttt attgctaaag aatatgttgc taagccctat gattaaatta
      601 ccatccttac tcaccacaag cgggatattg tatttagtgt ccggatcttc aataacgacc
      661 atttttaaat cctggctaga ttttaaaggt ttgatttcta aaatacgcac ctttttattg
      721 gtctgttttt caatcacgct caccaaatta tcctgcatat gtatatctcc ttcttaaagt
      781 taaacaaaaa ttatttctag agggggaatt gttatccgct cacaattccc ctatagtgag
      841 tcgtattaaa tttcgcgggg atcgagatct cgatcctcta cgccgggacg catcgtgggc
      901 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcacc
//
