LOCUS       sequence-53549-e        4890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E
  TITLE     A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive 
            Bacterial Immunity.
  JOURNAL   Science. 2012 Aug 17;337(6096):816-21. doi: 10.1126/science.1225829.
            Epub 2012 Jun 28.
  PUBMED    22745249
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1225829"; journalName: "Science"; date: 
            "2012-08-17- 17"; volume: "337"; issue: "6096"; pages: "816-21"
COMMENT     Sequence Label: pMJ824
FEATURES             Location/Qualifiers
     source          1..4890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..681
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     639..661
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             700..720
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgaaacatc accatcacca tcaccccatg agccctatac taggttattg gaaaattaag
       61 ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat
      121 ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag
      181 tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc
      241 atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag
      301 atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat
      361 agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa
      421 atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct
      481 gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat
      541 gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag
      601 tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt
      661 ggtggcgacc atcctccaaa aggatctggc agtggttctg agaatcttta ttttcagggc
      721 gccatggcta agccatactc aattggactt gatattggaa cgaatagtgt tggatgggct
      781 gtaacaactg ataattacaa ggttccgtct aaaaaaatga aagtcttagg aaatacgagt
      841 aaaaagtata tcaaaaagaa cctgttaggt gtattactct ttgactctgg aatcacagca
      901 gaaggaagaa gattgaagcg tactgcaaga agacgttata ctagacgccg taatcgtatc
      961 ctttatttgc aggaaatttt tagcacagag atggctacat tagatgatgc tttctttcaa
     1021 agacttgacg attcgttttt agttcctgat gataaacgtg atagtaagta tccgatattt
     1081 ggaaacttag tagaagaaaa agcctatcat gatgaatttc caactatcta tcatttaagg
     1141 aaatatttag cagatagtac taaaaaagca gatttgcgtc tagtttatct tgcattggct
     1201 catatgatta aatatagagg tcacttctta attgaaggag agtttaattc aaaaaataat
     1261 gatattcaga agaattttca agactttttg gacacttata atgctatttt tgaatcggat
     1321 ttatcacttg agaatagtaa acaacttgag gaaattgtta aagataagat tagtaaatta
     1381 gaaaagaaag atcgtatttt aaaactcttc cctggggaga agaattcggg gattttttca
     1441 gagtttctaa agttgattgt aggaaatcaa gctgatttta ggaaatgttt taatttagac
     1501 gaaaaagcct ccttacattt ttccaaagaa agctatgatg aagatttaga gactttgtta
     1561 ggttatattg gagatgatta cagtgatgtc tttctcaaag caaagaaact ttatgatgct
     1621 attcttttat cgggttttct gactgtaact gataatgaga cagaagcacc tctctcttct
     1681 gctatgataa agcgatataa tgaacacaaa gaagatttag cgttactaaa ggaatatata
     1741 agaaatattt cactaaaaac gtataatgaa gtatttaaag atgacaccaa aaatggttat
     1801 gctggttata ttgatggaaa aacaaatcag gaagatttct acgtatatct aaaaaaacta
     1861 ttggctgaat ttgaaggtgc ggattatttt cttgaaaaaa ttgatcgaga agattttttg
     1921 agaaagcaac gtacatttga caatggttcg ataccatatc agattcatct tcaagaaatg
     1981 agagcaattc ttgataagca agctaaattt tatcctttct tggctaaaaa taaagaaaga
     2041 atcgagaaga ttttaacctt ccgaattcct tattatgtag gtccacttgc gagagggaat
     2101 agtgattttg cctggtcaat aagaaaacga aatgaaaaaa ttacaccttg gaattttgag
     2161 gacgttattg acaaagaatc ttcggcagag gccttcatta atcgaatgac tagttttgat
     2221 ttgtatttgc cagaagagaa ggtacttcca aagcatagtc tcttatacga aacttttaat
     2281 gtatataatg aattaacaaa agttagattt attgccgaaa gtatgagaga ttatcaattt
     2341 ttagatagta agcagaagaa agatattgtt agactttatt ttaaagataa aaggaaagtt
     2401 actgataagg atattattga atatttacat gcaatttatg ggtatgatgg aattgaatta
     2461 aaaggcatag agaaacagtt taattctagt ttatctactt atcacgatct tttaaatatt
     2521 attaatgata aagagttttt ggatgatagt tcaaatgaag cgattatcga agaaattatc
     2581 catactttga caatttttga agatagagag atgataaaac aacgtctttc aaaatttgag
     2641 aatatattcg ataaatccgt tttgaaaaag ttatctcgta gacattacac tggctggggt
     2701 aagttatctg ctaagcttat taatggtatt cgagatgaaa aatctggtaa tactattctt
     2761 gattacttaa ttgatgatgg tatttctaac cgtaatttca tgcaacttat tcacgatgat
     2821 gctctttctt ttaaaaagaa gatacagaaa gcacaaatta ttggtgacga agataaaggt
     2881 aatattaaag aggtcgttaa gtctttgcca ggtagtcctg cgattaaaaa aggtatttta
     2941 caaagcataa aaattgtaga tgaattggtc aaagtaatgg gaggaagaaa acccgagtca
     3001 attgttgttg agatggctcg tgaaaatcaa tataccaatc aaggtaagtc taattcccaa
     3061 caacgcttga aacgtttaga aaaatctctc aaagagttag gtagtaagat acttaaggaa
     3121 aatattcctg caaaactttc taaaatagac aataacgcac ttcaaaatga tcgactttac
     3181 ttatactatc ttcaaaatgg aaaagatatg tataccggag atgatttaga tattgataga
     3241 ttaagtaatt atgatattga tcatattatt cctcaagctt ttttgaaaga taattctatt
     3301 gacaataaag tacttgtttc atctgctagt aaccgtggta aatcagatga tgttccaagt
     3361 ttagaggttg tcaaaaaaag aaagacattt tggtatcaat tattgaaatc aaaattaatt
     3421 tctcaacgaa aatttgataa tctgacaaaa gctgaacggg gaggattgtc acctgaggac
     3481 aaagctggtt ttattcaacg ccagttggtt gaaacacgtc aaataacaaa acatgtagct
     3541 cgtttacttg atgagaaatt taataataaa aaagatgaaa ataatagagc ggtacgaaca
     3601 gtaaaaatta ttaccttgaa atctacctta gtttctcaat ttcgtaagga ttttgaactt
     3661 tataaagttc gtgaaatcaa tgattttcat catgctcatg atgcttactt gaatgccgtt
     3721 gtagcaagtg ctttacttaa gaaataccct aaactagagc cagaatttgt gtacggtgat
     3781 tatccaaaat acaatagttt tagagaaaga aagtccgcta cagaaaaggt atatttctat
     3841 tcaaatatca tgaatatctt taaaaaatct atttctttag ctgatggtag agttattgaa
     3901 agaccactta ttgaggtaaa tgaggagacc ggcgaatccg tttggaataa agaatctgat
     3961 ttagcaactg taaggagagt actctcttat ccgcaagtaa atgttgtgaa aaaagttgag
     4021 gaacagaatc acggattgga tagaggaaaa ccaaagggat tgtttaatgc aaatctttcc
     4081 tcaaagccaa aaccaaatag taatgaaaat ttagtaggtg ctaaagagta tcttgacccc
     4141 aaaaagtatg gggggtatgc tggaatttct aattctttta ctgttcttgt taaagggaca
     4201 attgaaaaag gtgctaagaa aaaaataaca aatgtactag aatttcaagg tatttctatt
     4261 ttagatagga ttaattatag aaaagataaa cttaattttt tacttgaaaa aggttataaa
     4321 gatattgagt taattattga actacctaaa tatagtttat ttgaactttc agatggttca
     4381 cgtcgtatgt tggctagtat tttgtcaacg aataataaga ggggagagat tcacaaagga
     4441 aatcagattt ttctttcaca gaagtttgtg aaattacttt atcatgctaa gagaataagt
     4501 aacacaatta atgagaatca tagaaaatat gttgagaacc ataaaaaaga gtttgaagaa
     4561 ttattttact acattcttga gtttaatgag aattatgttg gagctaaaaa gaatggtaaa
     4621 ctcttaaact ctgcctttca atcttggcaa aatcatagta tagatgaact ctgtagtagt
     4681 tttataggac ctaccggaag tgaaagaaag gggctatttg aattaacctc tcgtggaagt
     4741 gctgctgatt ttgaattttt aggtgttaaa attccaaggt atagagacta taccccatca
     4801 tccctattaa aagatgccac acttattcat caatctgtta caggcctcta tgaaacacga
     4861 atagaccttg ccaaactagg agagggttaa
//
