LOCUS       sequence-53566-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   An SJ, Almers W
  TITLE     Tracking SNARE complex formation in live endocrine cells.
  JOURNAL   Science. 2004 Nov 5;306(5698):1042-6.
  PUBMED    15528447
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2004-11-5"; volume: "306(5698)"; pages: "1042-6"
COMMENT     Sequence Label: SCORE
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(57..59,60..62,63..773)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(102..123)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(363..382)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     710..731
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tttagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg
       61 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      121 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      181 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      241 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      301 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      361 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      421 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      481 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
      541 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      601 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      661 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      721 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggag
      781 gttctggtgg ttctggtggt tctggtggtt ctggtatggc cgaagacgca gacatg
//
