LOCUS       sequence-53567-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   An SJ, Almers W
  TITLE     Tracking SNARE complex formation in live endocrine cells.
  JOURNAL   Science. 2004 Nov 5;306(5698):1042-6.
  PUBMED    15528447
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2004-11-5"; volume: "306(5698)"; pages: "1042-6"
COMMENT     Sequence Label: SCORE
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(329..577)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELY"
     primer_bind     complement(368..389)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     717..736
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcat taaccacttc
      121 ccagcatctt tgttgcacgt tggttggctt catcaattct ggttttgttg gaatcagcct
      181 tctccatgat cctgtcgatc tggcgattct gggtgtcaat ctcattgccc atgtctagag
      241 ccatatggcg gaggtttccg atgatgccgc tcacctgctc taggttttca tccatttcat
      301 tttctcgaga accaccagaa ccaccactgt acagctcgtc catgccgaga gtgatcccgg
      361 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
      421 gggcggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
      481 gggtgttctg ctggtagtgg tcggcgagct gcacgccgcc gtcctcgatg ttgtggcgga
      541 tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc ggtgatatag acgttgtggc
      601 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      661 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      721 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
      781 cggacttgaa
//
