LOCUS       sequence-53572-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Conformation-Selective ATP-Competitive Inhibitors Control Regulatory
            Interactions and Noncatalytic Functions of Mitogen-Activated Protein
            Kinases.
  JOURNAL   Chem Biol. 2014 May 22;21(5):628-35. doi: 
            10.1016/j.chembiol.2014.02.016. Epub 2014 Apr 3.
  PUBMED    24704509
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.02.016"; journalName: "Chem Biol"; date: 
            "2014-05-22- 22"; volume: "21"; issue: "5"; pages: "628-35"
COMMENT     Sequence Label: pcDNA3-eGFP-LIC
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(73..75,76..78,79..591)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(118..139)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(379..398)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     725..746
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(838..856)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(839..856)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctcggnncnn tagtaacggc cgccagtgtg ctggaattct gcagatatcc atcacactgg
       61 cggccgctcg agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      121 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      181 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      241 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      301 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      361 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      421 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      481 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      541 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      601 gtgcagctcg ccgaccacta ccagcanaac acccccatcg gcgacggccc cgtgctgctg
      661 cccgacaacc actacctgag cacccagtcc gccctganca agaccccnac gannnncgcg
      721 atcacatggt cctgctggag ttcgtgaccg nccgccgggn tcactctcgg catggacgan
      781 ctgtacaagc agcttcnngg atcgtacttc caatccaata tggaantgna taactattct
      841 atagtgtcac ctaaatgcta nagctcgctg atcnncctcn actgtgcctt ctagttgcca
      901 gccatctgtt gttagccctn ccccgngcnt tccttgcccc ggaaaggngc cactcccnnn
      961 nnnctttcct ataaaatgag gaaattgcat cnnattgncn gagtaag
//
