LOCUS       sequence-53590-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG607
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             86..151
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(195..219)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgcccgggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattcg ccatggacta caaagaccat gacggtgatt ataaagatca
      121 tgacatcgat tacaaggatg acgatgacaa gcttgcggcc gcgaattcat cgatagatct
      181 gatatcggta ccgtacccag ctttcttgta caaagtggag atgaaagcgg catcaaatag
      241 tgtctcgtcg gccggaggat cagtgtcacc tacgacaacc cagccaccac taccaccagg
      301 gcagtcgtca catcctcaaa tctacgatca acaggtattc acaatcaaca aatgttgata
      361 atattacagt tatatttttc agatgcagta ctacttcgcg gctgccatgc cgaaccagcc
      421 aatggcgacg tatgcggcac aaaatggatc ttctcaacag tacgccccag cggctccata
      481 ttatcaagat gccaacggac agtatgtgca ggttcccgcg aacggatcaa tggctcctca
      541 acagcacatg atggtttctg gtcaaccgta tctctatatg gctcaaccac aacaaggagc
      601 tcagcaagtc atgcagtcag gacaaccaca gctcatctat tatcaacaat caatggctcc
      661 acaagcggct ccaatgtact tccatcctat gcaagccgca ccgatgcttc cagaacaaat
      721 gggtgtgatg ccacatacgc agccggcaat tccacctcaa caacagccaa gacaagtggg
      781 agtggagatt agtagcacac gcacagctc
//
