LOCUS       sequence-53591-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG608
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             86..151
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(195..219)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgcccgggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattcg ccatggacta caaagaccat gacggtgatt ataaagatca
      121 tgacatcgat tacaaggatg acgatgacaa gcttgcggcc gcgaattcat cgatagatct
      181 gatatcggta ccgtacccag ctttcttgta caaagtggag atgaaagcgg catcaaatag
      241 tgtctcgtcg gccggaggat cagtgtcacc tacgacaacc cagccaccac taccaccagg
      301 gcagtcgtca catcctcaaa tctacgatca acaggtattc acaatcaaca aatgttgata
      361 atattacagt tatatttttc agatgcaata ttacttcgct gccgcgatgc caaatcagcc
      421 gatggcaaca tacgcggcgc agaacggatc ttctcagcaa tatgcgccag ctgccccata
      481 ctatcaggat gcgaatggac aatatgttca agttcctgcc aacggttcta tggctcccca
      541 gcaacatatg atggtgtcag gacagccgta cctttatatg gctcagccac agcaaggtgc
      601 gcaacaagtg atgcaatcgg gacagcctca acttatttac tatcaacaga gtatggctcc
      661 acaagctgct ccaatgtatt tccacccaat gcaagcagcc ccaatgctcc cagaacaaat
      721 gggagtgatg ccgcatacgc aaccagctat ccctc
//
