LOCUS       sequence-53597-e        2048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Rose JC, Huang PS, Camp ND, Ye J, Leidal AM, Goreshnik I, Trevillian
            BM, Dickinson MS, Cunningham-Bryant D, Debnath J, Baker D, 
            Wolf-Yadlin A, Maly DJ
  TITLE     A computationally engineered RAS rheostat reveals RAS-ERK signaling 
            dynamics.
  JOURNAL   Nat Chem Biol. 2016 Nov 21. doi: 10.1038/nchembio.2244.
  PUBMED    27870838
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Nov 21. doi: 10.1038/nchembio.2244."
COMMENT     Sequence Label: Braf
FEATURES             Location/Qualifiers
     source          1..2048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..60
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     gap             1012..1024
                     /estimated_length=13
ORIGIN
        1 gnnnancttn nncttggtac cgagctcgga tccatggatt acaaggatga cgatgacaag
       61 ggaattctgt acttccaatc caatgcggcg ctgagcggtg gcggtggtgg cggcgcggag
      121 ccgggccagg ctctgttcaa cggggacatg gagcccgagg ccggcgccgg cgccggcgcc
      181 gcggcctctt cggctgcgga ccctgccatt ccggaggagg tgtggaatat caaacaaatg
      241 attaagttga cacaggaaca tatagaggcc ctattggaca aatttggtgg ggagcataat
      301 ccaccatcaa tatatctgga ggcctatgaa gaatacacca gcaagctaga tgcactccaa
      361 caaagagaac aacagttatt ggaatctctg gggaacggaa ctgatttttc tgtttctagc
      421 tctgcatcaa tggataccgt tacatcttct tcctcttcta gcctttcagt gctaccttca
      481 tctctttcag tttttcaaaa tcccacagat gtggcacgga gcaaccccaa gtcaccacaa
      541 aaacctatcg ttagagtctt cctgcccaac aaacagagga cagtggtacc tgcaaggtgt
      601 ggagttacag tccgagacag tctaaagaaa gcactgatga tgagaggtct aatcccagag
      661 tgctgtgctg tttacagaat tcaggatgga gagaagaaac caattggttg ggacactgat
      721 atttcctggc ttactggaga agaattgcat gtggaagtgt tggagaatgt tccacttaca
      781 acacacaact ttgtacgaaa aacgtttttc accttagcat tttgtgactt ttgtcgaaag
      841 ctgcttttcc agggtttccg ctgtcaaaca tgtggttata aatttcacca gcgttgtagt
      901 acagaagttc cactgatgtg tgttaattat gaccaacttg atttgctgtt tgtctccaag
      961 ttctttgaac accacccaat accacaggaa gaggcgtcct tagcagagac tnnnnnnnnn
     1021 nnnnaaacac tggtagacgg gactcgagtg atgatnggga gattccngat gggcagatta
     1081 cagtgggaca aagaattgga tctggatcat ttggaacagt ctacaaggga aagtggcatg
     1141 gtgatgtggc agtgaaaatg ttgaatgtga cagcacctac acntcagcag ttacaagcct
     1201 tcaaaaatga agtaggagta ctcaggaaaa cacgacatgt gaatatccta ctcttcatgg
     1261 gctattccac aaagccacaa ctggctattg ttacccagtg gtgtgagggc tccagcttgt
     1321 atcaccatct ccatatcatt gagaccaaat ttgagatgat caaacttata gatattgcac
     1381 gacagactgc acagggcatg gattacttac acgccaagtc aatcatccac agagacctca
     1441 agagtaataa tatatttctt catgaagacc tcacagtaaa aataggtgat tttggtctag
     1501 ctacagtgaa atctcgatgg agtgggtccc atcagtttga acagttgtct ggatccattt
     1561 tgtggatggc accagaagtc atcagaatgc aagataaaaa tccatacagc tttcagtcag
     1621 atgtatatgc atttggaatt gttctgtatg aattgatgac tggacagtta ccttattcaa
     1681 acatcaacaa cagggaccag ataattttta tggtgggacg aggatacctg tctccagatc
     1741 tcagtaaggt acggagtaac tgtccaaaag ccatgaagag attaatggca gagtgcctca
     1801 aaaagaaaag agatgagaga ccactctttc cccaaattct cgcctctatt gagctgctgg
     1861 cccgctcatt gccaaaaatt caccgcagtg catcagaacc ctccttgaat cgggctggtt
     1921 tccaaacaga ggattttagt ctatatgctt gtgcttctcc aaaaacaccc atccaggcag
     1981 ggggatatgg tgcgtttcct gtccactgac attggaagtg gataactcga gcnngcatct
     2041 agannnnc
//
