LOCUS       sequence-53598-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG587
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             309..329
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             342..386
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    complement(388..412)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggagtagtt taacttggac ttactaacta acggattata tttaaatttt cagaacatta
       61 acatggcact actactagaa ggtggaggtc actacctttg cgattttaaa actacttaca
      121 aagcaaagaa ggtcgtccaa cttccagatg cacactttgt tgatcacaga attgaaatac
      181 taggaaacga ttcggattac aacaaagtta agctatacga acacgcagtt gcaagataca
      241 gtcctctacc aagtcaagca tggtgcccgg gggatcggtg gagctccacc ggtggcggcc
      301 gctcgagaga gaatctttat tttcagggcg ccgccaaatt caaagaaacc gctgctgcta
      361 aattcgaacg ccagcacatg gacagccacc cagctttctt gtacaaagtg gagatgaaag
      421 cggcatcaaa tagtgtctcg tcggccggag gatcagtgtc acctacgaca acccagccac
      481 cactaccacc agggcagtcg tcacatcctc aaatctacga tcaacaggta ttcacaatca
      541 acaaatgttg ataatattac agttatattt ttcagatgca atattacttc gctgccgcga
      601 tgccaaatca gccgatggca acatacgcgg cgcagaacgg atcttctcag caatatgcgc
      661 cagctgcccc atactatcag gatgcgaatg gacaatatgt tcaagttcct gccaacggtt
      721 ctatggctcc ccagcaacat atgatggtgt caggacagcc gtacctttat atggctcagc
      781 cacagcaagg tgcgca
//
