LOCUS       sequence-53630-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz 
            L, Nusse R, Weissman IL
  TITLE     A role for Wnt signalling in self-renewal of haematopoietic stem 
            cells.
  JOURNAL   Nature. 2003 May 22. 423(6938):409-14.
  PUBMED    12717450
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 May 22. 423(6938):409-14."
COMMENT     Sequence Label: Lentiviral-FOP-dGFP-reporter
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(387..431)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    616..849
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc atcccatggt ggctttacca
       61 acagtaccgg aatgccaagc ttacttagat cgcagatctc gagtaccgga tccattatat
      121 accctctaga cctttggcct tacccccttt ggccttaccc cctttggccc tgcaggcatg
      181 caagcttcag ctgctcgagc ccgggctatg tcgggtcccc tcggggttgg gaggtgggtc
      241 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
      301 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
      361 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
      421 tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg
      481 taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga
      541 gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag
      601 caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt
      661 gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata
      721 gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc
      781 gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac
      841 aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg
      901 ggtgctactc ctaatgg
//
