LOCUS       sequence-53634-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of HPV 16E6no* in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pLentiN 16E6no*
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..39
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             63..86
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(607..631)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttctgcagat atcaacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca
       61 tggactacaa ggatgacgat gacaagctcg atggaggata cccatacgat gttccagatt
      121 acgctgatat cggatccttt caggacccac aggagcgacc cagaaagtta ccacagttat
      181 gcacagagct gcaaacaact atacatgata taatattaga atgtgtgtac tgcaagcaac
      241 agttactgcg acgcgagcta tatgactttg cttttcggga tttatgcata gtatatagag
      301 atgggaatcc atatgctgta tgtgataaat gtttaaagtt ttattctaaa attagtgagt
      361 atagacatta ttgttatagt ttgtatggaa caacattaga acagcaatac aacaaaccgt
      421 tgtgtgattt gttaattagg tgtattaact gtcaaaagcc actgtgtcct gaagaaaagc
      481 aaagacatct ggacaaaaag caaagattcc ataatataag gggtcggtgg accggtcgat
      541 gtatgtcttg ttgcagatca tcaagaacac gtagagaaac ccagctgtaa aagggtgggc
      601 gcgccgaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga
      661 gtctag
//
