LOCUS       sequence-53635-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of HPV 16E6no* in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pLentiN 16E6no*
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..36
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(518..544)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(557..580)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(604..628)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(724..748)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctggatatc aaccactttg tacaagaaag ctgggtcggc gcgcccaccc ttttacagct
       61 gggtttctct acgtgttctt gatgatctgc aacaagacat acatcgaccg gtccaccgac
      121 cccttatatt atggaatctt tgctttttgt ccagatgtct ttgcttttct tcaggacaca
      181 gtggcttttg acagttaata cacctaatta acaaatcaca caacggtttg ttgtattgct
      241 gttctaatgt tgttccatac aaactataac aataatgtct atactcacta attttagaat
      301 aaaactttaa acatttatca catacagcat atggattccc atctctatat actatgcata
      361 aatcccgaaa agcaaagtca tatagctcgc gtcgcagtaa ctgttgcttg cagtacacac
      421 attctaatat tatatcatgt atagttgttt gcagctctgt gcataactgt ggtaactttc
      481 tgggtcgctc ctgtgggtcc tgaaaggatc cgatatcagc gtaatctgga acatcgtatg
      541 ggtatcctcc atcgagcttg tcatcgtcat ccttgtagtc catggtgaag ggggcggccg
      601 cggagcctgc ttttttgtac aaacttgttg atatctgcag aattccacca cactggacta
      661 gtggatcctc tagagtcggt gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca
      721 ggcgatctga cggttcacta aacgagctct gcttatatag ac
//
