LOCUS       sequence-53640-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N HPyV7
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             43..66
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             79..105
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    121..145
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccatcctt ctctaggcgc cggaattaga tctgccacca tggactacaa ggatgacgat
       61 gacaagctcg atggaggata cccatacgat gttccagatt acgctgctcg aggttatcaa
      121 acaagtttgt acaaaaaagc aggcatggac aagcttttag gtagagatga agtcaaggag
      181 cttatggaat taataggcct gaacatggcc tgttggggga atttgcccct catacagcat
      241 aaggttagat tagccagcaa aaagtatcat ccagataaag gtggagatcc ccagaaaatg
      301 caaagactga atgtcttaaa agacaagctg caagctactt tgagagatca aaggtctgga
      361 agccccatgt ggcattacag ctctgatgag gtcagttttt gggatataga attgactgta
      421 ggggagtttt tggggccaga gtttaacaga aaaaaggtgt ggaactacaa tttgtgtgta
      481 gtgcaaggtt taagagcctg ctgttgtatt cattgcattt taaagagaaa gcataagaag
      541 aaggccaaag agtatgctaa agaccaccgg gggcctcttt tatggggcaa atgctggtgc
      601 tttgactgct atttagactg gtttggagta gaaagatctg aggaatcctt tatgtggtgg
      661 agccacatta ttttccaaac tccaatggat gtacttaacc tttggggaca gctaaatcta
      721 ctgtaagtaa tccaaggcct aaaagcctga ctaaggggag taaacaaagc aaccttacta
      781 attatctttt
//
