LOCUS       sequence-53644-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RFP with robust performance in protein fusions and useful as FRET 
            acceptor for Clover in a FRET pair that offers bright fluorescence, 
            dynamic range, and photostability while limiting emissions overlap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: pcDNA3-mRuby2
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..123
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..159
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(163..171,172..186)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgc ggccgccacc atggtgcggg gttctcatca tcatcatcat
      121 catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta cgacgatgac
      181 gataaggatc cgatggtgtc taagggcgaa gagctgatca aggaaaatat gcgtatgaag
      241 gtggtcatgg aaggttcggt caacggccac caattcaaat gcacaggtga aggagaaggc
      301 aatccgtaca tgggaactca aaccatgagg atcaaagtca tcgagggagg acccctgcca
      361 tttgcctttg acattcttgc cacgtcgttc atgtatggca gccgtacttt tatcaagtac
      421 ccgaaaggca ttcctgattt ctttaaacag tcctttcctg agggttttac ttgggaaaga
      481 gttacgagat acgaagatgg tggagtcgtc accgtcatgc aggacaccag ccttgaggat
      541 ggctgtctcg tttaccacgt ccaagtcaga ggggtaaact ttccctccaa tggtcccgtg
      601 atgcagaaga agaccaaggg ttgggagcct aatacagaga tgatgtatcc agcagatggt
      661 ggtctgaggg gatacactca tatggcactg aaagttgatg gtggtggcca tctgtcttgc
      721 tctttcgtaa caacttacag gtcaaaaaag accgtcggga acatcaagat gcccggtatc
      781 catgccgttg atcaccgcct ggaaaggtta gaggaaagtg acaatgaaat gttcgtagta
      841 caacgcgaac acgcagttgc caa
//
