LOCUS       sequence-53647-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG610
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             297..317
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             330..374
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    complement(376..400)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acttggactt actaactaac ggattatatt taaattttca gaacattaac atggcactac
       61 tactagaagg tggaggtcac tacctttgcg attttaaaac tacttacaaa gcaaagaagg
      121 tcgtccaact tccagatgca cactttgttg atcacagaat tgaaatacta ggaaacgatt
      181 cggattacaa caaagttaag ctatacgaac acgcagttgc aagatacagt cctctaccaa
      241 gtcaagcatg gtgcccgggg gatcggtgga gctccaccgg tggcggccgc tcgagagaga
      301 atctttattt tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc
      361 agcacatgga cagccaccca gctttcttgt acaaagtgga gatgaaagcg gcatcaaata
      421 gtgtctcgtc ggccggagga tcagtgtcac ctacgacaac ccagccacca ctaccaccag
      481 ggcagtcgtc acatcctcaa atctacgatc aacaggtatt cacaatcaac aaatgttgat
      541 aatattacag ttatattttt cagatgcaat attacttcgc tgccgcgatg ccaaatcagc
      601 cgatggcaac atacgcggcg cagaacggat cttctcagca atatgcgcca gctgccccat
      661 actatcagga tgcgaatgga caatatgttc aagttcctgc caacggttct atggctcccc
      721 agcaacatat gatggtgtca ggacagccgt acctttatat ggctcagcca cagcaaggtg
      781 cgcaacaagt gatgcaatcg ggacagcctc aacttattta ctatca
//
