LOCUS       sequence-53652-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     pFA6-yEGFP1-HIS3MX6
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRS413-GAL1-yEGFP1
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..740
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     primer_bind     complement(795..813)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gtggatcccc cgggttaatt aacatgtcta aaggtgaaga attattcact ggtgttgtcc
       61 caattttggt tgaattagat ggtgatgtta atggtcacaa attttctgtc tccggtgaag
      121 gtgaaggtga tgctacttac ggtaaattga ccttaaaatt tatttgtact actggtaaat
      181 tgccagttcc atggccaacc ttagtcacta ctttaactta tggtgttcaa tgtttttcta
      241 gatacccaga tcatatgaaa caacatgact ttttcaagtc tgccatgcca gaaggttatg
      301 ttcaagaaag aactattttt ttcaaagatg acggtaacta caagaccaga gctgaagtca
      361 agtttgaagg tgatacctta gttaatagaa tcgaattaaa aggtattgat tttaaagaag
      421 atggtaacat tttaggtcac aaattggaat acaactataa ctctcacaat gtttacatca
      481 tggctgacaa acaaaagaat ggtatcaaag ttaacttcaa aattagacac aacattgaag
      541 atggttctgt tcaattagct gaccattatc aacaaaatac tccaattggt gatggtccag
      601 tcttgttacc agacaaccat tacttatcca ctcaatctgc cttatccaaa gatccaaacg
      661 aaaagagaga ccacatggtc ttgttagaat ttgttactgc tgctggtatt acccatggta
      721 ttgatgaatt gtacaaataa ggcgcgccac ttctaaataa gcgaatgtcg acctcgagtc
      781 atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa
      841 aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta
      901 gtattaagaa cg
//
