LOCUS       sequence-53654-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Silvers R, Saxena K, Kudlinzki D, Schwalbe H
  TITLE     Recombinant expression and purification of human TATA binding 
            protein using a chimeric fusion.
  JOURNAL   Protein Expr Purif. 2012 Jul 24;85(1):142-147.
  PUBMED    22841618
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2012-07-24"; volume: "85(1)"; pages: "142-147"
COMMENT     Sequence Label: pRS-45
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    47..71
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             86..108
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             127..144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             154..171
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             406..426
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
       61 ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc
      121 agctctcatc accatcacca tcacagtagc ggtctggtgc cgcgtggtag ccacatgggc
      181 aaaaccaacc tggccaatga agatgaagcg tatgaagcca tttttggcgg tgaattcggt
      241 tctctggaaa ttggcagtta tatcggcggt gatgaaggcg cgaactctaa agattacacg
      301 gaacacctgc cggatgcagt tgattttgaa gatgaagatg aactggcgga tgatgatgat
      361 gatctgccgg gcggtggctc tggtggcggt agtggcggtg gcagcgagaa tctttatttt
      421 cagggccata tgagcggcat tgttccgcag ctgcagaata ttgtgagcac cgttaatctg
      481 ggttgcaaac tggatctgaa aaccattgcc ctgcgcgccc gcaacgcgga atataacccg
      541 aaacgcttcg cggcggttat tatgcgcatt cgcgaaccgc gtaccaccgc gctgattttt
      601 agcagcggta aaatggtgtg caccggcgcc aaaagcgaag aacagagccg tctggcggcc
      661 cgtaaatatg cgcgcgttgt tcagaaactg ggctttccgg cgaaatttct ggattttaaa
      721 attcagaaca tggtgggcag ctgcgatgtt aaatttccga ttcgtctgga aggcctggtt
      781 ctgacccatc agcagtttag cagctatgaa ccggaactgt ttccgggtct gatttatcgt
      841 atgatcaaac cgcgtattgt gctgct
//
