LOCUS       sequence-53664-e        9729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 9729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72114-BBa_J72100
FEATURES             Location/Qualifiers
     source          1..9729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             77..3151
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(97..113)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(106..128)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(3167..3261)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3282..3941)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3856..3875
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(4202..4219)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4336..4881)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4416..4435)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4961..4985
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      complement(8121..8148)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(8164..8235)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(8487..8771)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(8545..8564)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             8798..9676
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 gaattcatga gatctgttaa tgctagctca gtcctaggga ctctgctagc ggatctattt
       61 cactagggaa gccactatga ccatgattac ggattcactg gccgtcgttt tacaacgtcg
      121 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      181 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      241 gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct
      301 ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg
      361 ttacgatgcg cccatctaca ccaacgtgac ctatcccatt acggtcaatc cgccgtttgt
      421 tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct
      481 acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg
      541 caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat ttgacctgag
      601 cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg
      661 cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtctcgtt
      721 gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga
      781 tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc gtgactacct
      841 acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt
      901 cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa
      961 cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga
     1021 actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga
     1081 ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt
     1141 taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca
     1201 ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc attatccgaa
     1261 ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa
     1321 tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc gctggctacc
     1381 ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat
     1441 catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg
     1501 gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag ccgacaccac
     1561 ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc
     1621 tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat
     1681 cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta aatactggca
     1741 ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc
     1801 gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg attttggcga
     1861 tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc gcacgccgca
     1921 tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca
     1981 aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc tcctgcactg
     2041 gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc
     2101 acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg ccgggcaact
     2161 ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat
     2221 cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc
     2281 ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc tgggtaataa
     2341 gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgataaaaa
     2401 acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg
     2461 cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg
     2521 ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg ctgatgcggt
     2581 gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta tcagccggaa
     2641 aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtggcgag
     2701 cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg tagcagagcg
     2761 ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg
     2821 ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga
     2881 aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga
     2941 cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca
     3001 tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg
     3061 tggcgacgac tcctggagcc cgtcagtatc ggcggaattt cagctgagcg ccggtcgcta
     3121 ccattaccag ttggtctggt gtcaaaaata aggatccgag gcttggattc tcaccaataa
     3181 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     3241 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
     3301 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     3361 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     3421 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     3481 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     3541 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     3601 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     3661 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     3721 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     3781 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     3841 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     3901 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     3961 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     4021 ttggaacctc ttacgtgccc gatcaaagat ccgcaccgcc ggacatcagc gctagcggag
     4081 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
     4141 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
     4201 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     4261 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     4321 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     4381 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4441 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     4501 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     4561 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     4621 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     4681 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     4741 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
     4801 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
     4861 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctaaggc
     4921 ctcccctgat tctgtggata accgggatct gtaaggatca accactttgt acaagaaagc
     4981 tgggtcgaat tgagatccga acggtttatt acgtacatca ggtaaaactg accgataagc
     5041 cgctttcttt tgggtatagt gtcgtggaca gtcattcatc tttctgcccc tccaaaagta
     5101 aaaacccgcc gaagcgggtt tttacgtaaa acaggtgaaa ctgaccgata agccgctttc
     5161 ttttgggtat agtgtcgtgg acagtcattc atctttctgc ccctccaaaa gcaaaaaccc
     5221 gccgaagcgg gtttttacgt aaaccaggtg aaactgaccg ataagccgct ttcttttggg
     5281 tatagcgtcg tggacagtca ttcatctttc cgcccctcca aaagcaaaaa cccgccgaag
     5341 cgggttttta cgtaaatcag gtgaaactga ccgataagcc gggttctgtc gtggacagtc
     5401 attcatctag gccagcaatc gctcagatcc ttaatcatca tccccgtaca gtttgcgggt
     5461 aacttcttcg tcttcctcct cgtcttcgtc cagatcctgt tcaagccatg cttcgtttga
     5521 tatgccttcc gcatcgacat caccataacc acctgtatcg acgatatcgg caatttcttc
     5581 tctacgatgc tcaatccaca atgcggcatc agcgcggcgg ctggcggccc gttctcgcgc
     5641 gattttatcc agatcctcaa gtgatggagc gccagatgct gtttggtttt cctcatcatc
     5701 ggtattggtc ttaggagcac gcagatcggc tttgatttgc tccagcatca ggggcggcac
     5761 ttttcctcca tgcgcctcga tgaactcagc cgcttccagc actgaccagt tattttcacg
     5821 ctttcgttcg tatgccagct taacaatgcc agcttgcccc atagataaag cgtgcttttc
     5881 cgcctcccgg ctttcttttc gatagttatt ccggatgctg taaatggtgt tgatcaggct
     5941 gcttatctgc gcggaacagc tgtttagcat gctcgcgata cggtattcag gcggagtacc
     6001 ttcatcatcg tctttttgct gatcgcgcat ttcctgcacc aggcgaatac acgtatccct
     6061 ggcgttctcc agcataagga gatgagaaag agacttttcc agaagagtgg tttccagaac
     6121 atcggccccg gaccgacgca acatagcgcg cgcggccttc cgcgcttcaa cgttatctat
     6181 caggtaatcg ccaacttcga actcaaagcg ttcaccatca tcatccaggg tgtcgcgttc
     6241 caggcgatca cgtaaggtcc ggtgggcgcg ggtgatcacg tcatgatcat ctgaacgatc
     6301 atttatgcgc ttattttggc gcttcgcatt ctcgactgcg gcactgacaa cggcattaac
     6361 tctttgtttt tccgctattt cagccgcaat gtgatcacct gcatgttgat cattagagtg
     6421 atcaatgatc atgcttttta gtggcttcct gactggctta tttggcttgc ggctgtccgt
     6481 agtcctggtg tcttctttga aggcacggag ataacgacgt gcggtattag ggttaagatt
     6541 aaactcggcg gcatactgtg cgatggtgta accaccatct cgcgccaggc gagcaaaatt
     6601 cttcttgtga tcgtcccagg tcacttatgc ttcctttcga gatcccaaca atattcctac
     6661 tcaatgaaca aatgactact cgtagaatcg gttaacacac cagattcaga tccttaccct
     6721 ctgaatcctg ccggtatacc ccattgttcg ttatctttat ttttggctaa aaccgcatta
     6781 agagcttcgt ttaccgtcat gcaatgcggt aggttatcga agtttgatat cccgccaata
     6841 tcaggcgaac gcttgttttt caggtaagca tatttccgcg cagccgcctc tactttctgc
     6901 ttgaactcat gtttttgagt gcgttttttg gataaccgca gattgtcagc ctttgctttt
     6961 gccttagcga tccatgaagt caattttttg aggctggttg ttccggcacc gccggaaact
     7021 gatctttttg tttttttaac ttgtgacttc ttattcttta ttgccacgtc atcctgacag
     7081 ggggaggggg tatcattttg acatgggggt gtggataaaa aattaaataa agccaatgtc
     7141 ttagcgagaa cagctttaac cttggttgcc gctgacgaaa tctttaattt gctttctatc
     7201 agcgcatttt tggcttgttg tgcgaaggcc aaaaaggatg gtgtaaaccg gtacaggtta
     7261 gcgcgacgtt cacggtgatc gccgataaca atctctacag acagaatacc tttgtttaca
     7321 gcttcacgga atgcacgaac gacggttgat tggctataac cagtttctgc cgcgatcagg
     7381 cggtgaggct tgtgaatgaa gtattcactg gttgttgccg cgagatttgc acattgcgac
     7441 aggatatgcc cggcgctacg ggatagaccg gagtgtgtta caaagcaggc caattcatag
     7501 ccagaaaaag taaaatcgct ttaagatccc cgagttctct taaaccaagg tttaggattg
     7561 aaatgatgac gccggaaact tcttataaag cgtggaaaca gccacatcat agatgattgc
     7621 aacctgctta cgggggatgc ccttctccag caatcgccgc atttgctgcc atgtttcttc
     7681 ttggtattta ggccgacgcc cacctatacg accttctgcg cgagctgcat caagtccagc
     7741 gcgtgtacgt tcaacgataa gctcacgttc catttctgcc agcgccccca ttacgtgaaa
     7801 gaaaaagcgc cccattggtg tactggtgtc gatggagtca gtgagactcc ggaagttaat
     7861 gcctctgtca cgcagctctt ccaccagcac aactaagtga cgcatgctgc gcccaagacg
     7921 gtctaacttc catacgacca gggtatcacc tctggaaagt tacggagtac cttttttaac
     7981 ccagggcgct cagccttttt gccgctcgcc ttgtcctcaa aaattagctc acatcctgcg
     8041 ctttcaagag cgtttcgttg taaagcagtg ttttgttcat ttgttgatac gcgtacatag
     8101 cctattagag atcctataaa cgcagaaagg cccacccgaa ggtgagccag tgtgactcta
     8161 gtagagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
     8221 ctttcgtttt atttgatgcc tggagatcct tattgtgagt ctggctggtt agtggccatg
     8281 agatattcga ttgtgtcagt gagatcatcc aggtcgtctt gggtgatgcg gtactcctga
     8341 ttggatatct ttgagtagtg ttcagcaatg gcgcgggcag cgtcggtttc ggcggggtct
     8401 acagataaag cgttagagca atgtctaacg tcgtcgatgg ttggtggaat gaaagccata
     8461 attatgcctc actgtaagat ccgggtatgg agaaacagta gagagttgcg ataaaaagcg
     8521 tcaggtaaga tccgctaatc ttatggataa aaatgctatg gcatagcaaa gtgtgacgcc
     8581 gtgcaaataa tcaatgtgga cttttctgcc gtgattatag acacttttgt tacgcgtttt
     8641 tgtcatggct ttggtcccgc tttgttacag aatgctttta ataagcgggg ttaccggttt
     8701 ggttagcgag aagagccagt aaaagacgca gtgacggcaa tgtctgatgc aatatggaca
     8761 attggtttct tctctgaatg gcgggagtat gaaaagtatg gctgaagcgc aaaatgatcc
     8821 cctgctgccg ggatactcgt ttaatgccca tctggtggcg ggtttaacgc cgattgaggc
     8881 caacggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa
     8941 tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tttgccgacc
     9001 gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc
     9061 tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct
     9121 taactggccg tcaatatttg ccaatacggg gttctttcgc ccggatgaag cgcaccagcc
     9181 gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc
     9241 ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa
     9301 cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga
     9361 tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcttgtcgcc
     9421 gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga
     9481 ggaccaacgt atcagccagg cgaagctgct tttgagcacc acccggatgc ctatcgccac
     9541 cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgggtat ttaaaaaatg
     9601 caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc
     9661 cgtcaagttg tcataattgg taacgaatca gacaattgac ggctcgatgg agtagcatag
     9721 agatctcat
//
