LOCUS       sequence-53690-e         613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Chee MK, Haase SB
  TITLE     New and Redesigned pRS Plasmid Shuttle Vectors for Genetic 
            Manipulation of Saccharomycescerevisiae.
  JOURNAL   G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.
  PUBMED    22670222
REFERENCE   2  (bases 1 to 613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2012-05"; volume: "2(5)"; pages: "515-26. Epub 
            2012 May 1"
COMMENT     Sequence Label: pRSII405
FEATURES             Location/Qualifiers
     source          1..613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    14..121
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     30..46
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     31..47
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(81..97)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(81..97)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(134..154)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(134..152)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(173..189)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(173..189)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(186..208)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    197..213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(221..227,228..245,246..251))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    266..287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(404..421)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat
       61 cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg cggtggagct
      121 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
      181 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      241 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      301 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      361 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      421 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      481 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      541 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      601 agcatcacaa aaa
//
