LOCUS       sequence-53701-e         506 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Chee MK, Haase SB
  TITLE     New and Redesigned pRS Plasmid Shuttle Vectors for Genetic 
            Manipulation of Saccharomycescerevisiae.
  JOURNAL   G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.
  PUBMED    22670222
REFERENCE   2  (bases 1 to 506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2012-05"; volume: "2(5)"; pages: "515-26. Epub 
            2012 May 1"
COMMENT     Sequence Label: pRSII415
FEATURES             Location/Qualifiers
     source          1..506
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    15..122
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     31..47
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     32..48
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(82..98)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(82..98)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(135..155)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(135..153)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(174..190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(174..190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(187..209)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    198..214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(222..228,229..246,247..252))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    267..288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(405..422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatagggcga attgggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata
       61 tcgaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc gcggtggagc
      121 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
      181 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      241 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      301 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      361 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      481 ttatccacag aatcagggga taacgc
//
