LOCUS       sequence-53710-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Chee MK, Haase SB
  TITLE     New and Redesigned pRS Plasmid Shuttle Vectors for Genetic 
            Manipulation of Saccharomycescerevisiae.
  JOURNAL   G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.
  PUBMED    22670222
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2012-05"; volume: "2(5)"; pages: "515-26. Epub 
            2012 May 1"
COMMENT     Sequence Label: pRSII402
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(5..23)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        91..195
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(414..433)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
       61 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      121 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      181 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      241 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      301 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      361 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      421 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      481 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      541 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      601 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      661 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      721 cgtgacacca cgatgcctgt agca
//
