LOCUS       sequence-53715-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pCalN-ddGFP-A
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(90..113)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(288..307)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     557..575
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ggagctaccg ctgcctgtgc tacctgtacc atgccccagg tggtggcggc cctcggagcg
       61 ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttggtgtc
      121 cacgaagtag tagccgggca gttgcacggg cttcttggcc atgtagatgg tcttggactc
      181 caccaggttg aggccgccgt ccttcagctt caggcgcccc aggagctcgc ccttcagcac
      241 gccttcttcg gggtacaggc gctcggtggc ataatcccag cccaaggtct tcttctgcat
      301 tacggggccg tcggggggga agttggtgcc gcgcatcttc accttgtaga tcagcgtgcc
      361 gtcctgcagg gaggagtcct gtgtcgcggt caccagaccg ccgtcctcga agtgcatcac
      421 gcgctcccac ttgaagccct cggggaagga cagcttcatg taatcgggga cgtcggcggg
      481 gtgcttcacg tacatcttgg agccgtacat gatcaggggg gacaggatgt cccaggcgaa
      541 gggcaggggg ccgcccttgg tcaccttcag cttggcggtc tgggtgccct cgtaagggcg
      601 gccctcgccc tcgccctcga tctcgaactc gtggccgttc atggagccct ccaagcgcac
      661 cttgaagcgc atgaactctt tgatgacctc ctcgctcttg ctcaccatgg taccgaccgg
      721 tccagatccg ctgccgcctg acgcaccccc ctctcttcgt ggctttctgt ttcttggtga
      781 tctgttcaaa atttcatcct cctctgcttt aggttttctg tcttcctcct
//
