LOCUS       sequence-53716-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pTOM20-ddGFP-B
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(66..89)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(264..283)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     533..551
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctcgtccatg cccagacggt ggcggccctc ggagcgcccg tactgttcca cgatggtgta
       61 gtcctcgttg tgggaggtga tgtccagctt ggtgtccaca cagtaatcgc cgggcagttg
      121 cacgggcttc ttggccatgt agatggtctc gaactccacc aggtagtggc cgccgtcctt
      181 cagcttcagg gcctgatagc tatggccctt cagcacgccg tccttggggt acagcatctc
      241 ggtggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg gggggaagtt
      301 ggtgccgcga atcttcacct tgcagatcag cgtgccgtcc tgcagggagg agtcctgggt
      361 aacggtcacc agaccgccgt cttcgaagtt catcacgcgc tcccacttga agccctcggg
      421 gaagggcagc ttcttgtaat cggggatgtc ggcggggtgc ctcacgtacg ctttggagcc
      481 gtacatgatc tggggggaca ggatgtccca ggcaaagggc agggggccgc ccttggtcac
      541 cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc
      601 gaactcgtgg ccgttcatgg agccctccat gcgcaccttg aagcgcatga actctttgat
      661 ggtggccatg ttgttgtcct cggaggaggc ggtaccgacc ggtggatccc cgctaccgcc
      721 ttccacatca tcttcagcca agctctgagc acttacaatt ctctgactaa ttgttgggag
      781 cttagtcaga agcatctgga acactggtgg tggaagagtt tgctgtaaga cctgcagtaa
      841 ctgctgtggc tgtccacaca cagcaattgc at
//
